FastQCFastQC Report
Fri 10 May 2024
EGAF00006139869

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139869
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38582392
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT6943761.7997225262757166No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA4259121.1039025263130393No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC3062290.7937014376920953No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC2933390.7602924152551247No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG2667000.6912479661706822No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG2566890.6653008968443429No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC2214250.5739016906987001No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1838280.4764556847589958No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC1824690.4729333526029179No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1788330.4635093645826832No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC1763650.457112664243316No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1488230.38572776928916175No Hit
GCTTTGGCCATGCCGGTTCCCTGCTCTCTGTCGGCTCCGCTCTGAGATTG1374690.356299837501003No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG1372170.3556466898164323No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG1357590.3518677639271303No Hit
TTCTCCTCGGAGCAGGGCGGGCAGTGGATGGCTTCGTCGCCCAGGCTCGG1345360.3486979241722493No Hit
AGGAGCCTGCGCGGGACCCCAGCATCCTGAGGCTGCCCAGGGTCGTCGGG1335250.34607755786629296No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1306860.33871927899130777No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1288060.33384658991593885No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG1232400.3194203200257776No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA1208870.313321683113893No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT1165080.3019719461665311No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT1153150.2988798620883848No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC1153030.29884875981769093No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC1147210.29734029968903947No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG1120630.29045114673035305No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC1114970.2889841562959601No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC1095460.2839274454523193No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG1067260.27661841183926594No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA1045950.2710951669352175No Hit
TCGGCCACGACCCCTCGTACCACCTCGTCCACCAGCTGCAGCACCTCTTC992230.25717171708794No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT979680.2539189379445422No Hit
ACGCTGTGGCAGCTCTTGCCCTGCTCCACGCAGACGTCCAGCAGCGCCCC942800.24436017341796745No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC916560.23755914355958024No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG913600.23679195421579874No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT912620.23653795233846567No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT908870.23556600637928307No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG845260.21907921105565462No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT839190.21750595452972435No Hit
GGTCATACTCCTCCACCTGGCCGCTGGTCCACACCTTCACGCGGTCTGTG820790.2127369396900016No Hit
GTATGTCACTGCAGTCGGCGGCAGTGGCTGGGCGGATCACGAATTTAGCC769850.19953402578046484No Hit
AGGAGGACCCGGGAGCCCATCTCTTCCACTTGGTCCTGGCAGAGATGTAC751300.19472613310237477No Hit
CAGCACCTCTCCCTGGCCAGCACTTCTGGTCTCCACAGTGAACTCTGCCC745220.19315028472055334No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT744920.19307252904381875No Hit
TGCACCTGGCCCCGAAGCTTGTTGTGCAGCATGAGGATCTCCTCCTTGTC732540.18986381145057052No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG730620.18936617511946902No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC691550.17923979415273164No Hit
GCCGCGTCCTCCTTGCCCAGAAGGTAGGCCTTCACGGTGAGCGACGCCAT687120.17809160199295057No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC669840.17361287501303704No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT638700.16554183576798454No Hit
GCTTCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTT629660.1631987980423816No Hit
ATTCCTCGGGCGGAGTCCCACACGTGTTGGTGGCCACCACAGTCACGTTG614920.1593784024588211No Hit
ATGACCTCACAGCTCAAGTACACCTGGGCACCAGTGACATTCCAGATGTC604190.15659734108761325No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC604170.1565921573758309No Hit
CCAGCACGGCGTCCGACTCCTCGGTGATCTCCAGCAGCGCGTGCACGTCG600170.15555541501936945No Hit
CAAGCTGGGCCCGCGACTCGAAGGTGACTGGGATGGTGATCTCGTTGGAC597690.15491263475836337No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG595180.15426207892968377No Hit
AGGTCCCTGTGCACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATC585900.15185683666269317No Hit
GCCTCGTCAGATCCGTCTGGGCAGTCCCTCTCACCGTCGCACCGCCAGCC578860.150032170115321No Hit
ACCTTGCTGGGCTCCTCGGCGGCCTTGGTCTCGTCGCTGGCGGGCGGCTT568500.1473470074120858No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA550440.1426661156726623No Hit
GAAGGTCCTCTCGTACCTCCCCGCTGTCCTGGAGCAGTGATAGGTACCCA545900.1414894130980785No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA545380.14135463659173853No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT544460.14111618584975238No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA537390.13928374373470676No Hit
GCCCAGTGCCAGCTGGTAGTGGTCTACCTCACCGAGGAGGCGGAAGGTCG532060.13790228454472184No Hit
CGGCCAGGCACACGAAGTGCGGCATCACCGCCTGGTAACTGGAGCAGATG530680.13754460843174265No Hit
AGCGGAGGTCCTCCACGCCGTCATTCACCATGTCCACCAGGGCTGCCTCC516050.13375272326298485No Hit
AAGACAGCCAGGACGAGCAGCAGCCCGAACAGGACAGACCCTCCTTGGAT508860.13188917887724536No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG508330.1317518105150142No Hit
AACCCCACTCTGCTTCAGGTTACTTGCTGGCCCATCCACACGGTCAGATG507510.1315392783319396No Hit
ACCTCCTGCCAGATCTCCGTGACCTTGGCGTACATGTCTCGGATCCACTC505990.13114531623648423No Hit
AACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCT504130.13066323104072966No Hit
CTCCTCCGAGCGCTCCAGCTCTCCTTCCAGGATCACCAGCTTCCTGGCCA503470.13049216855191353No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG499680.12950985516916627No Hit
GCTGGTGGCCCCAGACCTGCCCATTGGCTTTGTGTCCTTGGCGCCGGTGG499100.12935952752747937No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA496810.12876599252840518No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG487830.1264385059381492No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG487740.12641517923512882No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC483400.12529031377836813No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA480280.12448165474032817No Hit
AGTTGTGCTCCCTCTGGGACTCCATCCTTCATCTCTCCAATCTCACCAGC473890.122825458825881No Hit
TTCCTGCTTCATGCGGGCCAGCTCTGTGCGGACATGCTCGTCGCTGCGCA473550.12273733572558178No Hit
GCGTCATCATCCACCACGGCCTGCTGTAGGGCCTGGTCATAGAACTGCTT472580.12248592570413985No Hit
TGGGAGGCTCAGGAGCTGGTTGTTTAGCTTCTCGGGCACGACGCTGAGGA472530.12247296642468408No Hit
GAAGGACGCCGCCCTGAGCAATGGTCACCCGGCCTAGCAGTTTGTTGAGC462980.11999774404863235No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT462620.11990443723655081No Hit
ACGCCGATGCCCCATTTTCTGTCTTCTGCCGTTTGGGATCCGCTTCATCC461100.11951047514109545No Hit
CCCGCACCGTGGAATGCGGCCATCTTGTCATCTGCCACCACCAGTGTCTC461040.11949492400574853No Hit
TCTTTGCCACTCCTGCCCCATTCATTGGCAGCCTGATCAGCCAGCGAGTC460380.11932386151693238No Hit
GGAGCTGAGCGAGGCGCGGGAGGCTGTGGCTGGGAGCGGCGCTGAGAGCT453800.11761842034055327No Hit
CATCCAGGATTCGGGTCACCTCGCTCTGCTGGGACTTGATTTCGCGGGAG449320.11645726890131644No Hit
ACACTCATCTGGACAACCCCTTCTTCCCAGCCTCGGATCACCTCCTGCTT446660.11576783523426958No Hit
CGCGCCGGATCTTGTACTCGTCGCTGTGCTTGTCCACGGTCTTCTTGGCC443450.11493584949320923No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT442520.11469480689533194No Hit
CTGAGGAGCTGGTTCTGGTGGAACTTGGAGCTGGGCAGGGTGACTGAGTG435960.11299454943073514No Hit
ACTTGCACATGGACAACGCCGCCTTCTTCTCCCGGAGCCAGCTCGAGTTG434520.11262132218240901No Hit
CGATCTTGACTTGCTCGCGGTAGCTCTTTTCGTTGAGGCAAACCCCGCGG428330.11101696338578489No Hit
GTCTTCACACCGTTCTCTGCCATCTCACACACAGACTTCAGGTAGGGATA423720.10982211781996305No Hit
CGGTTTGCTGCCCTCTCTCCGTCATCTGCGCTGAGGAGTTGTCCCGGGTG420820.10907047961152849No Hit
TCGATCATAGTCTTCCTGCCCTGCTGTGTCCCACAGAGCCAGCTCCACCT418350.10843029120641355No Hit
ACGTCATCCGTTGCCAGGTCTTCCTGCATGAGCTCACCAGGAACCTCTTC417880.10830847397952932No Hit
CCCAGCCGGCCACGTCGCAGAGAGTTCCCGGTGCCACGTCGCGGTCCACG415870.10778751094540744No Hit
CGGGCTCCGGGAGAGGGTCGAAGGTGAAGATCTCAGGACCGGAGCCCCGC412780.10698662747504095No Hit
AGCTCCTTGGTCAGGCTCTGCACCTGTTCCTCCAGCTGCTGCACCTTCTC412170.10682852426568057No Hit
GCACACTCATACTCATCACAGCACTGGGTCTTCCGAAGGGTGGGCAAACG399050.10342800933648696No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA398240.10321806900930351No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT395610.1025364109099301No Hit
CCACTTCTTGCTGTCGCCGCTCAATCTTTTCCCGTCTTTTCCTCTCCATG393470.10198175374922322No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC392100.10162666949213517No Hit
CTGGCAGCCCACTCCTCATCCTTCTCCACAACAGAGCTCCTCCTACAGCC388000.10056400857676216No Hit
CTCTCGTCGTCCTTGTCCAGCTCCTGGATCTCCTGGATGCTCTTCTGGGC387900.10053809001785063No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)