FastQCFastQC Report
Thu 9 May 2024
EGAF00006139875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80748195
Total Bases4.1 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC53

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG6871520.8509812510360139No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG5764510.7138871649081444No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG5749930.7120815517919626No Hit
AACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCT5604230.6940378048078969No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC5208570.6450385671159585No Hit
TGGGCTCAACTTTCTTGTCCACCTGGGTGTTGCTGGGCTTGTGATTCACG4615660.57161153880901No Hit
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT4394360.5442053534447426No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG4230830.5239535075675685No Hit
ATGTCTCGATCCCACTTAACTATCGTGGGCTGTGACAAAGTCACATGGTT4037480.5000086998848705No Hit
CTGACCTCAAGTGATCCGCCCGCCACGGCCTCCCAAAGTGCTGGGATTGT3614270.4475976212223691No Hit
CTGCCCACTCAGACTTTATTCAAACACCAGGAAGGGCCGGTGCAAGGAGG3516630.43550571006571726No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG3460500.4285544710937501No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT3444800.4266101551867506No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC3436680.4256045599533216No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG3347990.414621032705437No Hit
TGTGGGACTTCCACTGCTCAGGCGACAGGCTCAGATAGCTGCTGGCCGCG3124800.3869807863816646No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT3030120.37525544688645984No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA2933500.36328985434287414No Hit
TGGGCTCAACTTTCTTGTCCACCTCGGTGTTGCTGGGCTTGTGATTCACG2590050.3207563958550405No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT2549340.31571479709236844No Hit
GGCAGCTGTAGCTTTTGTGGGACTACCACTGCTCAGGCGTCAGGCTCAGG2456950.304273055267675No Hit
GTGTAGGAGGCCTGCAGGTACAAAATGACCAGGCTGTTGACGGCTGCCTC2456170.3041764586812126No Hit
TGGGCTCAACTTTCTTGTCCACCTAGGTGTTGCTGGGCTTGTGATTCACG2407280.29812183417846055No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT2221470.27511079349823236No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT2205950.2731887691111857No Hit
GTGGGGAACGAGCGCCGGGTTCCGACCAAGCACTGTTGAAGCAGGAAACC2104520.2606274976177486No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC2096340.2596144718776686No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG2066500.2559190332365943No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1990800.24654421067864118No Hit
AAGCGCACGGACTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCAC1944390.24079671378412856No Hit
GGTGAATCCAGAGGCTGCACAGGACAGTCTCAGGGACCTCCCAGGCTGGA1910120.23655265606865888No Hit
TCAGGGAGAACTGGTTCTTGGACGTGTCTACTGACATGGTGACTCGACTC1902090.23555820659520627No Hit
ATCTTCATGAGGTAGTCAGTCAGGACCCGGCCAGCCAGGTCCAGACGCAG1877110.2324646389928592No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1822070.22564838755838443No Hit
GATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGG1696270.210069091946885No Hit
CGTGTGTTGTAACCACTCTCCCATATGGCCAAACACATCCAGTTTGCTAG1690190.2093161339395884No Hit
CAGCCCCTCATGCTGTACATGGCAGGTGTATCTCTGCTCTTCTCCAGAAG1667350.20648758774112538No Hit
GCTTCTGAGGCTGTCTCCTTGGCAGGTGATCCTGACTGTCTGTCCCAAGG1550000.19195475514963523No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT1527090.1891175400267461No Hit
CTGACCTCAAGTGATCCGCCCGCCGCGGCCTCCCAAAGTGCTGGGATTGT1477130.18293040482205206No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC1472570.1823656863165796No Hit
ATGTCTCGATCCCACTTAACTATCCTGGGCTGTGACAAAGTCACATGGTT1468490.18186041186431473No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA1450500.1796324982868038No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1431640.17729684236285406No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC1407600.174319686031372No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC1393570.17258218589282398No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1386550.1717128166146624No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA1377410.17058090276816715No Hit
GGCTCCATCCTCTGGCTCGCGGCGACGCTGTCGAACCGCACGAACTGCGT1374410.17020937743561945No Hit
CTGACCTCAAGTGATCCGCCCGCCCCGGCCTCCCAAAGTGCTGGGATTGT1305180.16163581117819414No Hit
ATGTCTCGATCCCACTTAACTATCATGGGCTGTGACAAAGTCACATGGTT1287510.1594475269694883No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA1249620.15475516201941109No Hit
GGCTCCATCCTCTGGCTCGCGGCGGCGCTGTCGAACCGCACGAACTGCGT1157230.14331342019471766No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG1134950.14055422539166357No Hit
GGTCTTTCAAGGATTGTAGGTGGATAGTCCCTTGGTTGGTGCTGATGCAG1134630.14051459602285846No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1110920.13757830747795663No Hit
CCTCAGCCTGGAGCCCAGAGACGGACAGGGAGGCCGTGTTGCCAGACTTG1087130.1346321115908535No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT1048720.12987534891646804No Hit
TGTGGGACTTCCACTGCTCAGGCGGCAGGCTCAGATAGCTGCTGGCCGCG1043840.12927100104219047No Hit
TGGCCTCTCTGGGATAGAAGTTATACAGCAGGCACACAACAGAGGCAGTT1039720.128760772918825No Hit
AGTGCATGCCATAGCTACTGAAGGTGAATCCAGACGCTGCACAGGAGAGT1024930.12692915302936494No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC1016200.12584801431165116No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC973010.12049928794073973No Hit
ATGTAGGCTGTGTCTGTAGATGTGACCTCGGTCATGGTGACTCTGCCCTG965870.11961505764927624No Hit
TAGGAAATTTCACTCCGCTTCTCCAGCTCAATGGTCTCTTTGGTCGGCAG963510.11932279105433875No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA955140.1182862353765307No Hit
TAGTTGGAGCGCTTTGTCATGATTTCCAGGTTGGCTTTGTCCACAGCTAT938260.11619578617206243No Hit
TGTGACTCGTGCAGCCATCGACAGAGACGCTGTAGGTGAAGCGGCTGTTG903100.11184150927460361No Hit
TTGTTCTCATCCAGCTCGCACACCATGCCGTGTTTGCAGTGGTGGTTCTG897730.11117647892934326No Hit
GTCACCAAGTGGGGTTTTGAGCTCCACTCTCTTGTCCACCTTGGTGTTGC895890.110948610058714No Hit
AGGGACGCCATCTCTGCCAGGTTCACCAGCAGCTCCTCTGGGCCCTGCAG867850.10747608661716836No Hit
GCTTCTGAGGCTGTCTCCTTGGCAAGTGATCCTGACTGTCTGTCCCAAGG864220.10702654096478566No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC861120.10664263145448638No Hit
CTGCTGTCTGCTGATTGACAGTAAAAGTCAGCCTCGTCTTCTGCCTGGAC830370.10283449679587264No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)