FastQCFastQC Report
Fri 10 May 2024
EGAF00006139885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences38559722
Total Bases2 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT6967851.8070280693413714No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA4276291.1090043647098908No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC3081220.7990773377463666No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC2929730.7597902287781017No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG2675240.6937913089726114No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG2573370.6673725500406875No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC2203650.571490116033513No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1839690.4771014687294685No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC1830080.47460923084455847No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1782220.462197315634174No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC1761770.45689385416212286No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1485860.3853399150543668No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG1381160.3581872296693425No Hit
GCTTTGGCCATGCCGGTTCCCTGCTCTCTGTCGGCTCCGCTCTGAGATTG1378020.3573729084457611No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG1358450.35229766438668825No Hit
TTCTCCTCGGAGCAGGGCGGGCAGTGGATGGCTTCGTCGCCCAGGCTCGG1355920.35164153932437586No Hit
AGGAGCCTGCGCGGGACCCCAGCATCCTGAGGCTGCCCAGGGTCGTCGGG1342120.34806267534812624No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1311870.3402177017769993No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1286220.3335656828646223No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG1233180.3198103969732977No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA1215810.31530569644667045No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC1178550.30564276371079646No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT1161240.3011536234623268No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC1153010.29901927197504174No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT1148670.29789374518830813No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC1116350.2895119420207438No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG1107810.28729719576297774No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC1107470.287209020853418No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG1066510.27658653763115826No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA1041490.2700979016394361No Hit
TCGGCCACGACCCCTCGTACCACCTCGTCCACCAGCTGCAGCACCTCTTC991010.2570065209495027No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT983660.2551003868751958No Hit
ACGCTGTGGCAGCTCTTGCCCTGCTCCACGCAGACGTCCAGCAGCGCCCC949080.2461324798970283No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC919810.23854165753580903No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT916010.23755617325249387No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG913610.2369337621261896No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT911770.23645658026268968No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG846400.21950365720997678No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT844900.21911465025603657No Hit
GGTCATACTCCTCCACCTGGCCGCTGGTCCACACCTTCACGCGGTCTGTG825510.21408608703143658No Hit
GTATGTCACTGCAGTCGGCGGCAGTGGCTGGGCGGATCACGAATTTAGCC775770.20118661643878036No Hit
AGGAGGACCCGGGAGCCCATCTCTTCCACTTGGTCCTGGCAGAGATGTAC749090.19426747941803107No Hit
CAGCACCTCTCCCTGGCCAGCACTTCTGGTCTCCACAGTGAACTCTGCCC745830.19342203763813443No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG743510.19282037354937362No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT736000.1908727453999798No Hit
TGCACCTGGCCCCGAAGCTTGTTGTGCAGCATGAGGATCTCCTCCTTGTC728490.18892511725058597No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC709450.18398732231523868No Hit
GCCGCGTCCTCCTTGCCCAGAAGGTAGGCCTTCACGGTGAGCGACGCCAT687240.17822742601723113No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC667500.1731080945033784No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT635030.16468739064042007No Hit
GCTTCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTT630610.163541116816143No Hit
ATTCCTCGGGCGGAGTCCCACACGTGTTGGTGGCCACCACAGTCACGTTG611820.15866815637311907No Hit
CCAGCACGGCGTCCGACTCCTCGGTGATCTCCAGCAGCGCGTGCACGTCG608540.15781752783383657No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC605700.15708100800104316No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG600170.15564686903085037No Hit
CAAGCTGGGCCCGCGACTCGAAGGTGACTGGGATGGTGATCTCGTTGGAC597500.15495443665283687No Hit
ATGACCTCACAGCTCAAGTACACCTGGGCACCAGTGACATTCCAGATGTC596480.15468991192415754No Hit
AGGTCCCTGTGCACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATC586140.15200835732166326No Hit
GCCTCGTCAGATCCGTCTGGGCAGTCCCTCTCACCGTCGCACCGCCAGCC577300.14971580967310916No Hit
ACCTTGCTGGGCTCCTCGGCGGCCTTGGTCTCGTCGCTGGCGGGCGGCTT569810.14777336828310123No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA555740.14412448305514236No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA550600.14279148589297402No Hit
GAAGGTCCTCTCGTACCTCCCCGCTGTCCTGGAGCAGTGATAGGTACCCA547870.14208349323680292No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT545950.14158556433575947No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA540540.1401825459218819No Hit
GCCCAGTGCCAGCTGGTAGTGGTCTACCTCACCGAGGAGGCGGAAGGTCG531290.1377836697059175No Hit
CGGCCAGGCACACGAAGTGCGGCATCACCGCCTGGTAACTGGAGCAGATG530090.13747246414276534No Hit
AGCGGAGGTCCTCCACGCCGTCATTCACCATGTCCACCAGGGCTGCCTCC515000.13355905418612718No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG509800.13221049674580124No Hit
ACCTCCTGCCAGATCTCCGTGACCTTGGCGTACATGTCTCGGATCCACTC509560.1321482556331708No Hit
AACCCCACTCTGCTTCAGGTTACTTGCTGGCCCATCCACACGGTCAGATG508040.13175406191984476No Hit
AAGACAGCCAGGACGAGCAGCAGCCCGAACAGGACAGACCCTCCTTGGAT506410.13133134102989644No Hit
GCTGGTGGCCCCAGACCTGCCCATTGGCTTTGTGTCCTTGGCGCCGGTGG505850.1311861117670921No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG505380.13106422292152417No Hit
AACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCT505330.1310512560230595No Hit
CTCCTCCGAGCGCTCCAGCTCTCCTTCCAGGATCACCAGCTTCCTGGCCA505290.13104088250428778No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG494320.12819594498113862No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA493140.12788992617737233No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG489930.12705745129594037No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC480610.1246404214221254No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA479220.12427994164480749No Hit
AGTTGTGCTCCCTCTGGGACTCCATCCTTCATCTCTCCAATCTCACCAGC477750.12389871482994612No Hit
TGGGAGGCTCAGGAGCTGGTTGTTTAGCTTCTCGGGCACGACGCTGAGGA477340.12379238626253582No Hit
TTCCTGCTTCATGCGGGCCAGCTCTGTGCGGACATGCTCGTCGCTGCGCA476400.12354860857139997No Hit
GCGTCATCATCCACCACGGCCTGCTGTAGGGCCTGGTCATAGAACTGCTT474230.12298584517803318No Hit
CCCGCACCGTGGAATGCGGCCATCTTGTCATCTGCCACCACCAGTGTCTC466030.12085927382982688No Hit
GAAGGACGCCGCCCTGAGCAATGGTCACCCGGCCTAGCAGTTTGTTGAGC464610.12049101391343017No Hit
ACGCCGATGCCCCATTTTCTGTCTTCTGCCGTTTGGGATCCGCTTCATCC463470.12019536862843566No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT459610.11919432406696294No Hit
TCTTTGCCACTCCTGCCCCATTCATTGGCAGCCTGATCAGCCAGCGAGTC459310.1191165226761749No Hit
GGAGCTGAGCGAGGCGCGGGAGGCTGTGGCTGGGAGCGGCGCTGAGAGCT451030.11696920429042512No Hit
ACACTCATCTGGACAACCCCTTCTTCCCAGCCTCGGATCACCTCCTGCTT450680.11687843600117241No Hit
CATCCAGGATTCGGGTCACCTCGCTCTGCTGGGACTTGATTTCGCGGGAG450230.11676173391499037No Hit
CGCGCCGGATCTTGTACTCGTCGCTGTGCTTGTCCACGGTCTTCTTGGCC446640.11583071060522687No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT441540.11450808696183026No Hit
CTGAGGAGCTGGTTCTGGTGGAACTTGGAGCTGGGCAGGGTGACTGAGTG436730.11326067132952879No Hit
ACTTGCACATGGACAACGCCGCCTTCTTCTCCCGGAGCCAGCTCGAGTTG431610.1119328609267463No Hit
CGATCTTGACTTGCTCGCGGTAGCTCTTTTCGTTGAGGCAAACCCCGCGG430450.11163202888236591No Hit
CGGTTTGCTGCCCTCTCTCCGTCATCTGCGCTGAGGAGTTGTCCCGGGTG425690.11039758014852907No Hit
GTCTTCACACCGTTCTCTGCCATCTCACACACAGACTTCAGGTAGGGATA423410.10980628957854002No Hit
TCGATCATAGTCTTCCTGCCCTGCTGTGTCCCACAGAGCCAGCTCCACCT420760.10911904395991237No Hit
AGCTCCTTGGTCAGGCTCTGCACCTGTTCCTCCAGCTGCTGCACCTTCTC418400.10850700635237982No Hit
ACGTCATCCGTTGCCAGGTCTTCCTGCATGAGCTCACCAGGAACCTCTTC416340.10797277013563532No Hit
CCCAGCCGGCCACGTCGCAGAGAGTTCCCGGTGCCACGTCGCGGTCCACG415450.10774195934296414No Hit
CGGGCTCCGGGAGAGGGTCGAAGGTGAAGATCTCAGGACCGGAGCCCCGC409720.10625595277891267No Hit
GCACACTCATACTCATCACAGCACTGGGTCTTCCGAAGGGTGGGCAAACG402130.10428757759197538No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA397490.10308424941445377No Hit
CCACTTCTTGCTGTCGCCGCTCAATCTTTTCCCGTCTTTTCCTCTCCATG396120.10272895639652174No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT393860.10214285258591854No Hit
CTCTCGTCGTCCTTGTCCAGCTCCTGGATCTCCTGGATGCTCTTCTGGGC388670.10079688852528554No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC387020.10036898087595135No Hit
CTGGCAGCCCACTCCTCATCCTTCTCCACAACAGAGCTCCTCCTACAGCC387010.10036638749625841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)