FastQCFastQC Report
Wed 8 May 2024
EGAF00006139887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24594367
Total Bases1.2 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT4642871.887777798875653No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA3282781.3347690550441895No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC2358040.9587723888156992No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG1799250.7315699566490164No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG1761200.7160989343616772No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC1596020.6489372139563503No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1410360.5734483835261953No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1334730.5426974396210319No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1312530.5336709824652125No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC1174200.47742639605239684No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC1098190.4465209452229448No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG1075010.43709602284132787No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG1063640.4324730130277393No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC1052150.42780121155384887No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT980810.3987945695044723No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG949770.3861737933730923No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC935170.38023747470304886No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC916150.3725039965452252No Hit
GCTTTGGCCATGCCGGTTCCCTGCTCTCTGTCGGCTCCGCTCTGAGATTG912740.37111750019831774No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA910570.3702351843411949No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA893700.36337589009711047No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG873820.35529273837379105No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC871350.3542884433659138No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC857100.34849443370508376No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG837410.3404885354439088No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC831520.33809367811743235No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG806980.3281157835857292No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA785980.319577243032927No Hit
AGGAGCCTGCGCGGGACCCCAGCATCCTGAGGCTGCCCAGGGTCGTCGGG764580.3108760636124524No Hit
TTCTCCTCGGAGCAGGGCGGGCAGTGGATGGCTTCGTCGCCCAGGCTCGG755860.30733053629719354No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT728530.29621823566347527No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT688590.2799787447263839No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT642720.2613281325760488No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG633510.2575833726478913No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT614750.24995560975405465No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT605850.24633689494834324No Hit
ACGCTGTGGCAGCTCTTGCCCTGCTCCACGCAGACGTCCAGCAGCGCCCC589770.23979881246791188No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG581130.23628581292618753No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC579480.23561492759703878No Hit
TCGGCCACGACCCCTCGTACCACCTCGTCCACCAGCTGCAGCACCTCTTC554360.22540120670721062No Hit
ATGACCTCACAGCTCAAGTACACCTGGGCACCAGTGACATTCCAGATGTC543860.22113193643080956No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT533920.21709036056914982No Hit
GTATGTCACTGCAGTCGGCGGCAGTGGCTGGGCGGATCACGAATTTAGCC498380.20263989717645509No Hit
AGGAGGACCCGGGAGCCCATCTCTTCCACTTGGTCCTGGCAGAGATGTAC497900.20244473053524814No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC486600.19785018252350223No Hit
CAGCACCTCTCCCTGGCCAGCACTTCTGGTCTCCACAGTGAACTCTGCCC482050.19600016540372842No Hit
GGTCATACTCCTCCACCTGGCCGCTGGTCCACACCTTCACGCGGTCTGTG474630.192983214408405No Hit
ACACTCATCTGGACAACCCCTTCTTCCCAGCCTCGGATCACCTCCTGCTT472650.1921781520134265No Hit
TGCACCTGGCCCCGAAGCTTGTTGTGCAGCATGAGGATCTCCTCCTTGTC465200.18914900310302762No Hit
TCTTTGCCACTCCTGCCCCATTCATTGGCAGCCTGATCAGCCAGCGAGTC451510.18358268785693896No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC450980.18336719135727297No Hit
CAAGCTGGGCCCGCGACTCGAAGGTGACTGGGATGGTGATCTCGTTGGAC426170.17327951558989096No Hit
GCCGCGTCCTCCTTGCCCAGAAGGTAGGCCTTCACGGTGAGCGACGCCAT414900.16869716549322045No Hit
GGGTCCACCTTCCCAGGGAAACCATGGCTTCAGCAACCAGTTTGTTCTCC414840.1686727696630696No Hit
GCTTCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTT398850.16217128092786448No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA395550.16082951026956702No Hit
CTGAGGAGCTGGTTCTGGTGGAACTTGGAGCTGGGCAGGGTGACTGAGTG387190.15743035793521337No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG384990.15653584416301505No Hit
GTCTTCACACCGTTCTCTGCCATCTCACACACAGACTTCAGGTAGGGATA379330.1542345041854503No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT378450.15387669867657092No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA375660.1527422925745558No Hit
CCAGCACGGCGTCCGACTCCTCGGTGATCTCCAGCAGCGCGTGCACGTCG375390.1526325113388769No Hit
ATTCCTCGGGCGGAGTCCCACACGTGTTGGTGGCCACCACAGTCACGTTG367240.14931874441005127No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC365550.1486315951941353No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT364030.1480135674969801No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG362740.14748905714873653No Hit
GCCTCGTCAGATCCGTCTGGGCAGTCCCTCTCACCGTCGCACCGCCAGCC361170.14685069959312227No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT358500.14576508515140887No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA356360.1448949672093614No Hit
GCGTCATCATCCACCACGGCCTGCTGTAGGGCCTGGTCATAGAACTGCTT355330.1444761721251049No Hit
CATGCCTGGAAGACCTGTCCACACTGGTAGCCAACCATGAGGCTGTACTC354550.1441590263331437No Hit
GAAGGTCCTCTCGTACCTCCCCGCTGTCCTGGAGCAGTGATAGGTACCCA348220.1415852662522276No Hit
ACCTTGCTGGGCTCCTCGGCGGCCTTGGTCTCGTCGCTGGCGGGCGGCTT347260.14119493296981378No Hit
ACCTCCTGCCAGATCTCCGTGACCTTGGCGTACATGTCTCGGATCCACTC347100.1411298774227448No Hit
AGGTCCCTGTGCACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATC344310.13999547132072965No Hit
GCCCAGTGCCAGCTGGTAGTGGTCTACCTCACCGAGGAGGCGGAAGGTCG341740.13895051659593433No Hit
CATCCAGGATTCGGGTCACCTCGCTCTGCTGGGACTTGATTTCGCGGGAG325230.13223759733275509No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG324670.1320099029180137No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG323840.13167242726759343No Hit
AACCCCACTCTGCTTCAGGTTACTTGCTGGCCCATCCACACGGTCAGATG321690.13079824335385415No Hit
CGGCCAGGCACACGAAGTGCGGCATCACCGCCTGGTAACTGGAGCAGATG318760.12960691364815366No Hit
AGCGGAGGTCCTCCACGCCGTCATTCACCATGTCCACCAGGGCTGCCTCC312800.12718359451983458No Hit
ACTTGCACATGGACAACGCCGCCTTCTTCTCCCGGAGCCAGCTCGAGTTG308660.12550028223942497No Hit
ACGCCGATGCCCCATTTTCTGTCTTCTGCCGTTTGGGATCCGCTTCATCC306970.124813133023509No Hit
GCTGGTGGCCCCAGACCTGCCCATTGGCTTTGTGTCCTTGGCGCCGGTGG304330.123739716496871No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG303820.12353235194058867No Hit
TTCCTGCTTCATGCGGGCCAGCTCTGTGCGGACATGCTCGTCGCTGCGCA296370.12050320303018978No Hit
ACGTCATCCGTTGCCAGGTCTTCCTGCATGAGCTCACCAGGAACCTCTTC293790.11945418233370267No Hit
CGCGCCGGATCTTGTACTCGTCGCTGTGCTTGTCCACGGTCTTCTTGGCC290910.11828318248646122No Hit
CTCCTCCGAGCGCTCCAGCTCTCCTTCCAGGATCACCAGCTTCCTGGCCA290310.1180392241849526No Hit
CCCGCACCGTGGAATGCGGCCATCTTGTCATCTGCCACCACCAGTGTCTC287520.11690481808293744No Hit
TGGGAGGCTCAGGAGCTGGTTGTTTAGCTTCTCGGGCACGACGCTGAGGA283570.11529875926467228No Hit
TCGATCATAGTCTTCCTGCCCTGCTGTGTCCCACAGAGCCAGCTCCACCT283400.1152296377459115No Hit
GAAGGACGCCGCCCTGAGCAATGGTCACCCGGCCTAGCAGTTTGTTGAGC280340.11398545040821748No Hit
CGGGCCTCGTTCACACGCTCTCTGTCATTGCTGTCCACCACGAAGATCAG278950.11342028034305579No Hit
CCCAGCCGGCCACGTCGCAGAGAGTTCCCGGTGCCACGTCGCGGTCCACG278240.11313159635293724No Hit
AGCTCCTTGGTCAGGCTCTGCACCTGTTCCTCCAGCTGCTGCACCTTCTC276520.11243224922194582No Hit
AACTTCATCCACGTTCACCTTGCCCCACAGGGCAGTAACGGCAGACTTCT275360.1119605965056958No Hit
AGTTGTGCTCCCTCTGGGACTCCATCCTTCATCTCTCCAATCTCACCAGC274540.11162718682696732No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA274370.11155806530820656No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG266790.10847605876581415No Hit
GCACACTCATACTCATCACAGCACTGGGTCTTCCGAAGGGTGGGCAAACG266530.10837034350182706No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC265070.10777671163482272No Hit
GAATCAATGTCCAGGCGGCACATGTGCTCCAGGAATGTGTCAGCCATGGC263900.10730099294688088No Hit
AGAGGAAGCCATCGCTGCGGCCGGTATCAGTGCAGCTGTTGTACTCCTTG263660.10720340962627743No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT260380.10586977091136356No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC254870.10362942050917594No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC252500.10266578521821684No Hit
AGCATCTGTTCCAGCTGCTGCATCTTCTGCCTGGTCACTGACTGGTTGTT252320.10259259772776425No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT251450.10223885819057672No Hit
GGAGCTGAGCGAGGCGCGGGAGGCTGTGGCTGGGAGCGGCGCTGAGAGCT248230.10092961530581371No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)