FastQCFastQC Report
Sat 11 May 2024
EGAF00006139893

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139893
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53555626
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT16937583.1626145122456415No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT6925941.2932236101581558No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA3657600.6829534585217993No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG2837150.5297576019370962No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG2834660.5292926647893165No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG2798440.5225296031457087No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT2584090.4825057968699685No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG2027570.3785914107324598No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA1991070.37177606699994503No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG1969950.36783250372239135No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA1913890.3573648826362332No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC1897570.3543175837399417No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC1773880.3312219709652913No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT1638220.30589129888986827No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC1550880.2895830215858181No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1508670.2817014966830936No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC1408820.26305733033537876No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA1406710.2626633474511156No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT1372310.25624011938540314No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC1365010.2548770506389002No Hit
GTGGGCATCCGGGCTAAGGCTTTACTTGGCGGCAGTCTCATCGGATTTTG1318620.2462150288374932No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG1224520.22864451252983206No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG1223620.2284764629583454No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT1204370.22488206934599178No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC986490.18419913530653156No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC983110.1835680158047261No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC956510.1786012173585647No Hit
GGGTAACCGAAATTGGAGAGCATGTCGGTCCAGAGACACTCGTTCTTGGA936570.17487798574140465No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC906710.16930247440296936No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC905790.16913069039656078No Hit
TAGACACGGACTCGGTAGTTGGACTTGGTCTCCTTTCCATTCCTGTTCAT903900.16877778629643878No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC879960.16430766769489352No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA864210.16136680019387692No Hit
GGTCATACTCCTCCACCTGGCCGCTGGTCCACACCTTCACGCGGTCTGTG857970.16020165649823606No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT836760.1562412882635337No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC806810.15064897196794974No Hit
GCAGCAGAAAGCCCGTCATCTCGGCGCTTTCCCTTAACCGAGTCGCTCTG806740.150635901445723No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC790730.14764648629072136No Hit
GCTTGTGGGAGATTTTCATCTTCTTGGTACAGCCGCTGTAGATCTCTTCA768300.1434583175257815No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT764630.14277304871760813No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG755110.14099545769477143No Hit
GCAAGGAGATGGCAACAGCCTTCTTCTGCATCTTCCCCATCCAGATCTTC735870.13740293129987874No Hit
GAGTGAGCAGACACTCGGGAGGTGTCTTGAGTTTATCATCCGCTGAGGGT735610.13735438364589372No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC733980.13705002719975676No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG722920.13498488468793177No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA722070.13482617120374993No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA714840.1334761729794737No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT704960.13163136212804236No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG702510.13117389385010642No Hit
GGCGTTGCCGCCTTTGCCACAAGATGACCCCAATGAGCAGGGCGGCTGTC697680.13027202781646133No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT697120.13016746363864742No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG683960.1277102054600202No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC679340.12684755099305534No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC666430.1244369732509522No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG662330.12367141409195741No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA649940.12135793165782434No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG643470.12014984196058133No Hit
ACCAAAGATCACCCGGCCTACATCTTCATCTCCAATTCGTAGGTCAAAAT637090.11895855722048698No Hit
TACCAGAGCTGCTCCTTTTACTGCTTCCACCAGCCAGGGCTCTAGAGGTC636970.11893615061095542No Hit
AAGACAGCCAGGACGAGCAGCAGCCCGAACAGGACAGACCCTCCTTGGAT634640.11850108894255107No Hit
GAGGATTGGGGTTCAGAGCACTTCTGGGGTGTCAGCTCCTCCTTGTCCCC630230.1176776460422664No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC627710.11720710724210374No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT622940.11631644451322444No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA610280.11395254720764539No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG606750.11329341944392546No Hit
CTGTTGAAGAGGTCGGCGAAGAGCTCGTCGTGGCACAGCTCCAGGGTGGG603570.11269964429133925No Hit
CTTCTCGTTCAATTTCATCCTTCCTTTTCTCCAGTTCTTCCTCCACCCTT597670.11159798598937111No Hit
TTCTGCGACACGGGCACAGACGGGTCGTAAAACTCGCGGACGACAATGTT576690.10768056375627091No Hit
TGGCACCAAACACCACTCCAGTCTTCTTAATGTCTCTCCAGTACAGGAGG572130.10682911259407182No Hit
CCCACGGCTATGGGGAAATTAGCCCGAGGCTTAGCTTTCATTATCACTGT569470.10633243274945567No Hit
AGCCTCTGGATGGCCCGGTTCATCTCTGAAATCTCATTCCGGGTATTCCG559800.10452683346470454No Hit
TCGGCCACGACCCCTCGTACCACCTCGTCCACCAGCTGCAGCACCTCTTC556130.10384156465653113No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC547920.10230857912108059No Hit
TTCGAGGCAAGGAGAAGCAGGAGTTCCCGATCAAGAGGAAAACACGCAGC546310.10200795710986554No Hit
GCACACTCATACTCATCACAGCACTGGGTCTTCCGAAGGGTGGGCAAACG544810.10172787449072111No Hit
AGTTGTGCTCCCTCTGGGACTCCATCCTTCATCTCTCCAATCTCACCAGC539340.10070650653957439No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)