FastQCFastQC Report
Fri 10 May 2024
EGAF00006139895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14410773
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT2530231.755790615812212No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG2226691.545156529771165No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1894981.3149745679846598No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG1836261.274227274275988No Hit
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT1709561.1863069385660296No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG1319040.9153152297937105No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT1236440.8579969998833512No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG1126660.7818178802760962No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC1117480.7754476460076084No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC1015030.7043549988609216No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG960340.6664042241176098No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG951250.6600964431262639No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA908990.6307711598815691No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT818880.5682415509563574No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG787650.5465702637880703No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC629980.43715906148823525No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG597970.414946512584717No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT577660.4008528897096637No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC518550.35983496513337626No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC457630.31756103576123224No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA457610.31754715725520066No Hit
ATGGCCTGCACCTCGCCGCGGTACTGCACCAGGCGGCCGCACACGTCCTC456010.31643687677267557No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC449360.3118222735171805No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC444100.30817222643087916No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT429430.29799234225672694No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG429410.2979784637506954No Hit
CATTCAGGATCGCAGACCAGCTCATCAGCGAGGCAGCCACCGAGTTGGCC425870.29552196818310855No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG422970.2935095848085318No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA421220.2922952155307699No Hit
TCCATGCCCAGTAGTGAGTTCTTGTCGGTGGCCTGCAGGAAGACGTTCTC412820.28646624299751305No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC400630.27800729357127474No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG396390.2750650502925832No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA388890.26986061053074667No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA386050.26788986267426457No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC371760.25797367011471206No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC371690.25792509534360164No Hit
CTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA361570.25090257129163024No Hit
GATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGG358430.24872364584467466No Hit
GAGAGTCTCAGGGATCCCCCAGGCTGTACCAAGCCTCCCCCAGACTCCAC353930.24560098198757274No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG352430.24456009403520546No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG335330.23269397137821823No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA332950.23104242916046214No Hit
GGTCAATCCGGAGAGTCCAGTTGCTCCCAGTGACTGCAGAGTAGCCACAA328460.227926704556376No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC317860.22057109635964706No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA309830.2149988761879741No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG307280.2132293666689497No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT298720.20728936608744025No Hit
CACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACA291060.20197389827735124No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT282580.19608941171996813No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT278660.19336922453778155No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC272510.1891015839330756No Hit
TTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAATATCGACACC267190.18540990132867957No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT258110.17910905959034953No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG252250.17504265732310126No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT248900.1727180075628143No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA246650.17115667563426334No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT245270.17019905871808544No Hit
CAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAG239150.16595223587242683No Hit
TCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGTGCTG236990.16445335722101792No Hit
ACCTGCTCTTGTCCACGGTGAGCCTGCTGTAGAGGAAGAAGGAGCCGTCG234050.162413216834378No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG232670.16145559991820008No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC224310.15565438439700632No Hit
TAGTTGGAGCGCTTTGTCATGATTTCCAGGTTGGCTTTGTCCACAGCTAT221100.1534268841789403No Hit
GTGCACAGGAGCAGAGGGCCATGGTGCAGACGAGGACAAGGAGGGCAGCT218630.15171288868404215No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC218550.15165737465991588No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC208380.14460015434286558No Hit
GTGATGTTGGCGGTTAGTGGGGTCTTCAACTCGGGGTGGGCAGCAGTGCA208250.1445099440536604No Hit
CATGCTGGAGATTGTCCTGAGTCCTGTCTTCTCTACCCACAGCTGAAGCT206010.14295555137812524No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC204940.14221305130543657No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA199650.13854218646008787No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT198840.13798010696580953No Hit
GTGCTTCATAGTAGAGGGTCACATTGACAAGCGGTGCATTGGACTTCTTC197940.13735557419438915No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC188470.13078410158844359No Hit
GGCTCCACCTCAAGGGTGATGGTCTTGCCGGTAAGGGTTTTCACGAAGAT187310.12997914823861287No Hit
AGGAGCTGCAGAGGGTCAGGATGCTCAGGCAGAAGTCCAGCAGCTGCAGC185370.12863293315355115No Hit
CCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTG182070.12634297965834312No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC180900.12553108705549662No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA179060.12425426450059272No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC174920.12138141375205896No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC168480.11691253480989536No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT166610.1156148944959441No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC163730.1136163896273989No Hit
CCTGCACACCACACCCACAGTGGTTTCAGACATGCTACTCTTGCCAACAG162240.11258244092804738No Hit
TGCAAATCCAGCAAGAAGTCCCAGTCTTTTCTGTTCAGTTTCATGGCTAT158800.11019533789061838No Hit
CAGCACCAGTTCAAGAAGAGGGTGTTGAACAGGGACCAGACGACATGGTC156790.10880054803444617No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG155390.1078290526122367No Hit
AGCCTCGATGCACCCTGAGCCAGGTGTTCTCCAGGGTGTGCTGACTGCAG151560.1051713187071922No Hit
CCATCAGGAGTCTGCCAGGTGACTTCACACGTGTAGTGGCTCCGGTCATC150880.10469944950211901No Hit
GGCGTTGTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGT150360.10433860834529833No Hit
CGGTGACCACAGCTCCAAGGACAGCTAGGACAACCAGGACAGCCAGGCCA150140.10418594477895114No Hit
AGCTCCAGGATCTCGGCGGTCAGGTACTCGAGGACCGCCGCCATGTAGAC148840.10328384188689947No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG148400.10297851475420507No Hit
AAGCGCACGGACTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCAC147540.1023817389948478No Hit
CGGCTGCGCTTGCGCTTCTTGCCATCTTTCTTCTGCGCTTTGGTCACTGC145580.10102164540375454No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)