FastQCFastQC Report
Fri 10 May 2024
EGAF00006139899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102014204
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT28429852.78685211326062No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT14797361.4505195766660102No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT13714161.3443382845000682No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT12884761.263035880768133No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC11770061.153766783300098No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA10035830.9837679074572793No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG9398180.921261905842053No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG8471890.8304618051031403No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG7345500.7200467887785509No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC5113270.5012311814931183No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC4917640.4820544401836434No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT4831370.4735977746785144No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC4798180.4703443061713249No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA4650600.45587769326710625No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA4606130.451518496385072No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG4437160.43495511664238445No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC4043960.3964114644270518No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG3986060.3907357842051093No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG3678430.3605801795993036No Hit
ACTCGTGAAGTCAGCCTGAAACTGTCTGAACATGTCGGTCATTCCCAGGT3171670.3109047442060127No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG2797070.2741843675023921No Hit
GGGTCCACCTTCCCAGGGAAACCATGGCTTCAGCAACCAGTTTGTTCTCC2650520.2598187209302736No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA2487360.24382486972108317No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC2461300.24127032349338332No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC2381840.23348121208689723No Hit
TCACACACAGACTTCAGGTAGGGATACTGGTCCTTTGTACTGAGATAGGC2248850.2204447921781559No Hit
CCCACGGCTATGGGGAAATTAGCCCGAGGCTTAGCTTTCATTATCACTGT2215990.21722367210746454No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA2098440.2057007669245745No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA2081720.20406177947533657No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC2073860.2032912985332905No Hit
TCCTCAGAAAGTGGGAAGATACGATTCAAGTCCTGGATTCTCGTCTTTGG2071750.20308446459083285No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC2060750.20200618337422893No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA2016680.19768619671825305No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1960580.1921869625135731No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG1778680.17435611221355019No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG1777990.17428847457359958No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG1727050.169295052285072No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC1671360.16383600856210181No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC1663610.16307631043222176No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT1659900.1627126355855308No Hit
TTGTCCTCCTTTTCAGAATACTTTTCAGATGCAGCCTCCAGAGATTTCAG1635780.16034825895421387No Hit
GTCCTTCGACATAATCCTCATAGGTGCCCTGGTCCTTGTTCTTGGCCACT1568900.15379230915726205No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC1484950.1455630629632713No Hit
GCTTCCTGCGTCTCAAAGTGTACAAATCCATAGCCCTTGGAACCATTTTC1475380.14462495830482586No Hit
ATTGATCTTCTCGGTCTTGCATCATACTCCTGTAGGCGGTGTCTGTGATG1475110.1445984914022365No Hit
AACCGCAGTACTTGGGCCGGTATTTCTTCACACTCAAACATCCAGCGTAA1471280.14422305348772804No Hit
GGAGCTGAGCGAGGCGCGGGAGGCTGTGGCTGGGAGCGGCGCTGAGAGCT1456590.1427830579357361No Hit
TTCGAGGCAAGGAGAAGCAGGAGTTCCCGATCAAGAGGAAAACACGCAGC1450080.14214491150663686No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG1419970.1391933617400965No Hit
AAGACAGCCAGGACGAGCAGCAGCCCGAACAGGACAGACCCTCCTTGGAT1384800.13574580261391833No Hit
CATTCCACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCC1370600.13435383958884786No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC1357470.13306676391848335No Hit
TTGCCATCTCTACACTGGTCCAGGTCCTTCATTATTTTGTCCACAGCCAG1325500.1299328866007718No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG1308270.12824390611330946No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1302200.1276488909328744No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG1259710.12348378466982891No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC1245430.12208397959954675No Hit
GAGTGAGCAGACACTCGGGAGGTGTCTTGAGTTTATCATCCGCTGAGGGT1235140.12107529653419635No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA1202890.11791397205824397No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1190950.11674354681040298No Hit
CCAAAAGCGCCAATCCTGTTTGCCATATCTCTGCCTTCTTCAGGTTTCAC1183840.1160465850422163No Hit
CAGGAACAGGCCCCGAAGAAGCGCTCCCTGCTCAGGACGCGCCACCACAT1174240.1151055396168165No Hit
AGAGCCAGAACGAAACTCCCTCATAATCACGTCTCGTTCCTTTTGGTCCA1171040.1147918578083499No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC1167450.11443994602947645No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1166670.11436348608866272No Hit
CTCTTGCTGGGGCTGGTGGTCCAGGGGTCTTACTCCTTGGAGGCCATGTG1159240.11363515613962935No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1136600.1114158573447282No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT1131320.11089828236075831No Hit
TGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAAC1099330.10776244453174384No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1082000.10606366148776694No Hit
AGGAGCTGCAGAGGGTCAGGATGCTCAGGCAGAAGTCCAGCAGCTGCAGC1074730.1053510156291569No Hit
CCTGCACACCACACCCACAGTGGTTTCAGACATGCTACTCTTGCCAACAG1071880.10507164276849135No Hit
ACAGCAAGGAGTAGTTGCTCTAAATTGCCAGAAGTCTCGCGGTCAATGGT1053650.10328463671588323No Hit
GCAGCAGAAAGCCCGTCATCTCGGCGCTTTCCCTTAACCGAGTCGCTCTG1049520.10287979113183102No Hit
AATGGCCAATTGAAACAAACAGTTCTGAGACCGTTCTTCCACCACTGATT1029170.10088497088111377No Hit
CGCGCCGGATCTTGTACTCGTCGCTGTGCTTGTCCACGGTCTTCTTGGCC1029030.10087124730199334No Hit
ACCATCTGATTTCTCATAGCTGGTTATAGCTGCCTTTCGCACTTGGATCT1027850.1007555771351213No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)