FastQCFastQC Report
Sat 11 May 2024
EGAF00006139909

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139909
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53542997
Total Bases2.6 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT16946563.1650376238745093No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT6941681.296468331796967No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA3667080.684885084038161No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG2845560.5314532542883246No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG2828270.5282240738223899No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG2811020.5250023639879553No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT2579040.48167643660290443No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG2017290.37676075547283994No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA1992390.3721102873640039No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG1980180.3698298770985868No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA1908720.3564835939235901No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC1903540.35551614714432217No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC1785280.3334292251141639No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT1644910.30721291152230423No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC1549890.2894664263937261No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1516990.28332183198486255No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA1412280.26376558637537606No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC1407070.26279253662248303No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC1372250.25628935190161284No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT1368090.2555124062255985No Hit
GTGGGCATCCGGGCTAAGGCTTTACTTGGCGGCAGTCTCATCGGATTTTG1330260.24844705648434284No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG1233470.23036999591188367No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG1226970.22915601829311122No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT1204410.22494258212703336No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC980890.18319669330426164No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC979820.18299685391163292No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC963920.18002727789032802No Hit
GGGTAACCGAAATTGGAGAGCATGTCGGTCCAGAGACACTCGTTCTTGGA933940.17442803958097453No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC907010.16939843692350653No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC901570.16838243103948775No Hit
TAGACACGGACTCGGTAGTTGGACTTGGTCTCCTTTCCATTCCTGTTCAT892100.16661375903183007No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC882700.1648581606292976No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA867410.16200251173836983No Hit
GGTCATACTCCTCCACCTGGCCGCTGGTCCACACCTTCACGCGGTCTGTG857930.1602319720728371No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT840730.1570196005277777No Hit
GCAGCAGAAAGCCCGTCATCTCGGCGCTTTCCCTTAACCGAGTCGCTCTG807290.15077415259366225No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC806230.1505761808589086No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC791810.1478830182031088No Hit
GCTTGTGGGAGATTTTCATCTTCTTGGTACAGCCGCTGTAGATCTCTTCA768300.14349215453890263No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT760960.1421212936586273No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG756350.14126030337823636No Hit
GCAAGGAGATGGCAACAGCCTTCTTCTGCATCTTCCCCATCCAGATCTTC748500.13979419194633427No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC740050.1382160210419301No Hit
GAGTGAGCAGACACTCGGGAGGTGTCTTGAGTTTATCATCCGCTGAGGGT732440.1367947333990288No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG723190.13506714986462187No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA721670.13478326586761663No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA715260.1335860971697195No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT714650.1334721700393424No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG693600.12954075021239472No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT691570.12916161566376272No Hit
GGCGTTGCCGCCTTTGCCACAAGATGACCCCAATGAGCAGGGCGGCTGTC690840.1290252766388852No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG687980.1284911264866253No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC675740.12620511324758305No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG665780.12434492600404867No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC664000.12401248290229251No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA650120.1214201737717446No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG646020.12065443404298046No Hit
ACCAAAGATCACCCGGCCTACATCTTCATCTCCAATTCGTAGGTCAAAAT638870.11931905866233077No Hit
AAGACAGCCAGGACGAGCAGCAGCCCGAACAGGACAGACCCTCCTTGGAT634060.11842071522443916No Hit
TACCAGAGCTGCTCCTTTTACTGCTTCCACCAGCCAGGGCTCTAGAGGTC632740.11817418438493459No Hit
GAGGATTGGGGTTCAGAGCACTTCTGGGGTGTCAGCTCCTCCTTGTCCCC632270.11808640446480798No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT629570.11758213683854865No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC623580.11646340977140297No Hit
CTTCTCGTTCAATTTCATCCTTCCTTTTCTCCAGTTCTTCCTCCACCCTT608740.11369180548485175No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG608650.11367499656397642No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA604680.11293353638758771No Hit
CTGTTGAAGAGGTCGGCGAAGAGCTCGTCGTGGCACAGCTCCAGGGTGGG600150.11208748737019708No Hit
TTCTGCGACACGGGCACAGACGGGTCGTAAAACTCGCGGACGACAATGTT581990.10869582066913439No Hit
TGGCACCAAACACCACTCCAGTCTTCTTAATGTCTCTCCAGTACAGGAGG581560.1086155113805079No Hit
CCCACGGCTATGGGGAAATTAGCCCGAGGCTTAGCTTTCATTATCACTGT565570.10562912643832768No Hit
AGCCTCTGGATGGCCCGGTTCATCTCTGAAATCTCATTCCGGGTATTCCG560630.10470650344806064No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC555360.10372224774791744No Hit
TTCGAGGCAAGGAGAAGCAGGAGTTCCCGATCAAGAGGAAAACACGCAGC555160.10368489459041674No Hit
TCGGCCACGACCCCTCGTACCACCTCGTCCACCAGCTGCAGCACCTCTTC552320.10315447975390694No Hit
GCACACTCATACTCATCACAGCACTGGGTCTTCCGAAGGGTGGGCAAACG545650.10190875195125891No Hit
AGTTGTGCTCCCTCTGGGACTCCATCCTTCATCTCTCCAATCTCACCAGC544690.10172945679525561No Hit
GAAGACACCAGGTTCTCCAGCTCCTCCAGTCTGCTCAGCAGCTCCTTAAC536900.1002745513106037No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)