FastQCFastQC Report
Fri 10 May 2024
EGAF00006139911

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139911
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102642539
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT28588942.7852915836386316No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT14898681.4515112491517772No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT13780051.342528169534076No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT12950771.261735156415022No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC11752441.144987264977925No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA10090190.9830417386693834No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG9443300.920018161281065No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG8522360.830295127442239No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG7398240.7207771818660877No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC5134200.5002019679189736No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC4948630.4821227191194092No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT4874860.47493564047553416No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC4812960.46890500243763455No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA4679930.4559444890582841No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA4625180.4506104432977832No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG4440400.4326081606379593No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC4062330.3957745043699669No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG4000870.38978673354913795No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG3692000.3596949214204454No Hit
ACTCGTGAAGTCAGCCTGAAACTGTCTGAACATGTCGGTCATTCCCAGGT3180230.3098354766925631No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG2844000.2770781030660202No Hit
GGGTCCACCTTCCCAGGGAAACCATGGCTTCAGCAACCAGTTTGTTCTCC2666470.2597821552329293No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA2524070.24590876498095982No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC2472980.24093129652609235No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC2381580.23202660643458944No Hit
TCACACACAGACTTCAGGTAGGGATACTGGTCCTTTGTACTGAGATAGGC2278020.22193722234404195No Hit
CCCACGGCTATGGGGAAATTAGCCCGAGGCTTAGCTTTCATTATCACTGT2232620.21751410494629328No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA2095290.20413466194557014No Hit
TCCTCAGAAAGTGGGAAGATACGATTCAAGTCCTGGATTCTCGTCTTTGG2086990.20332603035082755No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC2083230.20295971049585979No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA2082600.20289833243505406No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC2073700.20203124554430593No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA2034460.19820826918554693No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1959210.19087700081152512No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG1791000.17448905857638614No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG1788970.17429128482490092No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG1732360.16877602764678298No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC1690070.1646559035333294No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC1663030.16202151819334867No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT1657100.16144378501782775No Hit
TTGTCCTCCTTTTCAGAATACTTTTCAGATGCAGCCTCCAGAGATTTCAG1638580.15963946488112496No Hit
GTCCTTCGACATAATCCTCATAGGTGCCCTGGTCCTTGTTCTTGGCCACT1571800.153133390435714No Hit
ATTGATCTTCTCGGTCTTGCATCATACTCCTGTAGGCGGTGTCTGTGATG1491220.14528284418217674No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC1476420.143840946880708No Hit
GCTTCCTGCGTCTCAAAGTGTACAAATCCATAGCCCTTGGAACCATTTTC1476320.14383120433137375No Hit
AACCGCAGTACTTGGGCCGGTATTTCTTCACACTCAAACATCCAGCGTAA1475440.14374546989723236No Hit
GGAGCTGAGCGAGGCGCGGGAGGCTGTGGCTGGGAGCGGCGCTGAGAGCT1465680.14279459708220976No Hit
TTCGAGGCAAGGAGAAGCAGGAGTTCCCGATCAAGAGGAAAACACGCAGC1463450.142577338232056No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG1430630.13937983354055575No Hit
AAGACAGCCAGGACGAGCAGCAGCCCGAACAGGACAGACCCTCCTTGGAT1392120.13562797779193672No Hit
CATTCCACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCC1379150.13436436914328476No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC1365890.13307250710156343No Hit
TTGCCATCTCTACACTGGTCCAGGTCCTTCATTATTTTGTCCACAGCCAG1330160.12959149422443653No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG1317790.12838634087179002No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1308390.12747054123437068No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG1269920.12372258250548536No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC1247380.12152661188554582No Hit
GAGTGAGCAGACACTCGGGAGGTGTCTTGAGTTTATCATCCGCTGAGGGT1242460.1210472784583008No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA1217600.11862528069380669No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1204270.1173265988675514No Hit
CCAAAAGCGCCAATCCTGTTTGCCATATCTCTGCCTTCTTCAGGTTTCAC1184460.11539659984443683No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC1180780.11503807402893648No Hit
CAGGAACAGGCCCCGAAGAAGCGCTCCCTGCTCAGGACGCGCCACCACAT1180480.11500884638093374No Hit
AGAGCCAGAACGAAACTCCCTCATAATCACGTCTCGTTCCTTTTGGTCCA1173870.11436486386993992No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1161900.11319868071463042No Hit
CTCTTGCTGGGGCTGGTGGTCCAGGGGTCTTACTCCTTGGAGGCCATGTG1152050.11223903960520697No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1147610.11180647041476634No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT1139780.11104362880189471No Hit
TGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAAC1106710.1078217677370588No Hit
CCTGCACACCACACCCACAGTGGTTTCAGACATGCTACTCTTGCCAACAG1086110.10581480257420366No Hit
AGGAGCTGCAGAGGGTCAGGATGCTCAGGCAGAAGTCCAGCAGCTGCAGC1079170.10513866965040683No Hit
ACAGCAAGGAGTAGTTGCTCTAAATTGCCAGAAGTCTCGCGGTCAATGGT1065190.10377666125347892No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1044170.10172877738341994No Hit
GCAGCAGAAAGCCCGTCATCTCGGCGCTTTCCCTTAACCGAGTCGCTCTG1041500.10146865131619554No Hit
ACCATCTGATTTCTCATAGCTGGTTATAGCTGCCTTTCGCACTTGGATCT1038240.10115104420789904No Hit
AATGGCCAATTGAAACAAACAGTTCTGAGACCGTTCTTCCACCACTGATT1035390.10087338155187295No Hit
CTGAGGAGCTGGTTCTGGTGGAACTTGGAGCTGGGCAGGGTGACTGAGTG1028590.10021088819714408No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)