FastQCFastQC Report
Wed 8 May 2024
EGAF00006139913

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139913
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26568300
Total Bases1.9 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT5620212.11538186485398No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5324792.0041892029222796No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG4349681.6371691075454584No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC3359711.2645558805042099No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG3309531.2456687104556934No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG2481820.934128265639879No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG2170110.8168042366278611No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT2139610.8053243903448847No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC1989290.7487456856479338No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC1744580.6566396796181916No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG1717360.6463943872961386No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG1711420.6441586401839786No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1592800.5995114478532687No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT1490550.561025733675094No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1451100.5461772111877689No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1125460.4236100917258538No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC1065860.4011773429237099No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1050080.3952379339287798No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1019870.3838672402825924No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC899510.3385651321311488No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT849020.3195612816777889No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC828340.3117775695095283No Hit
ATGGCCTGCACCTCGCCGCGGTACTGCACCAGGCGGCCGCACACGTCCTC801330.3016113187520466No Hit
CATTCAGGATCGCAGACCAGCTCATCAGCGAGGCAGCCACCGAGTTGGCC797190.3000530707647836No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA789750.2972527410485428No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC786200.2959165622188849No Hit
GATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGG781660.2942077588705337No Hit
TCCATGCCCAGTAGTGAGTTCTTGTCGGTGGCCTGCAGGAAGACGTTCTC773260.2910460962876812No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA762010.28681172675707517No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA757540.2851292705969144No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC753530.283619953102005No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG747760.2814481920183075No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG735910.27698798944606917No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG725220.27296439742098666No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA706700.26599368420260233No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG691780.26037796923401196No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC652770.24569505764388389No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC646250.243241005258146No Hit
CACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACA628700.23663538879040058No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA620150.23341726794714No Hit
GAGAGTCTCAGGGATCCCCCAGGCTGTACCAAGCCTCCCCCAGACTCCAC617430.23239349149174016No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC592460.2229950730758084No Hit
GGTCAATCCGGAGAGTCCAGTTGCTCCCAGTGACTGCAGAGTAGCCACAA582380.2192010779763854No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA578150.21760895503287753No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG570150.21459784781111324No Hit
CTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA569090.21419887610422947No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG529010.19911322892319042No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT524590.19744959218316568No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT519390.19549237248901885No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT518650.19521384507100567No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT509100.19161933582502455No Hit
ACCTGCTCTTGTCCACGGTGAGCCTGCTGTAGAGGAAGAAGGAGCCGTCG488790.18397488736577047No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC481410.18119714095369294No Hit
TCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGTGCTG472040.17767038162020152No Hit
TTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAATATCGACACC471750.17756122898341256No Hit
CAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAG465750.17530289856708933No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT451570.16996571101651217No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG449280.16910378157428213No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC435250.16382305228411304No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT433790.16327352521614105No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA431700.16248687345445512No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG424620.1598220435631937No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC417090.1569878388907081No Hit
GTGCACAGGAGCAGAGGGCCATGGTGCAGACGAGGACAAGGAGGGCAGCT402030.15131942954573685No Hit
GTGATGTTGGCGGTTAGTGGGGTCTTCAACTCGGGGTGGGCAGCAGTGCA399610.15040856961115312No Hit
TAGTTGGAGCGCTTTGTCATGATTTCCAGGTTGGCTTTGTCCACAGCTAT393010.1479244061531976No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC381600.1436298144781563No Hit
CATGCTGGAGATTGTCCTGAGTCCTGTCTTCTCTACCCACAGCTGAAGCT380500.14321578723516373No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC377660.1421468441714374No Hit
GTGCTTCATAGTAGAGGGTCACATTGACAAGCGGTGCATTGGACTTCTTC368020.13851845996921144No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC364500.13719357279163513No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC358690.13500675617182883No Hit
CCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTG348650.13122781660851465No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT345490.13003842925591777No Hit
GGCTCCACCTCAAGGGTGATGGTCTTGCCGGTAAGGGTTTTCACGAAGAT344870.12980506844623105No Hit
AGGAGCTGCAGAGGGTCAGGATGCTCAGGCAGAAGTCCAGCAGCTGCAGC341470.12852534787698122No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA335920.12643639224188225No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC334640.12595461508639996No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC334340.1258416985655838No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA331050.12460338072063323No Hit
CGGCTGCGCTTGCGCTTCTTGCCATCTTTCTTCTGCGCTTTGGTCACTGC301550.11349992284037744No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT298830.11247614638497758No Hit
TGCAAATCCAGCAAGAAGTCCCAGTCTTTTCTGTTCAGTTTCATGGCTAT294390.11080498187689841No Hit
CAGCACCAGTTCAAGAAGAGGGTGTTGAACAGGGACCAGACGACATGGTC292340.11003338565132133No Hit
GGCGTTGTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGT285380.1074137223683864No Hit
CCTGCACACCACACCCACAGTGGTTTCAGACATGCTACTCTTGCCAACAG285240.1073610279920055No Hit
GCTTTGGCCATGCCGGTTCCCTGCTCTCTGTCGGCTCCGCTCTGAGATTG283220.10660072341851003No Hit
AGCTCCAGGATCTCGGCGGTCAGGTACTCGAGGACCGCCGCCATGTAGAC280700.10565222464365429No Hit
CTGCGCTTGCGCTTCTTGCCGTCCTTCTTCTGCACTTTCGTAACAGCCTT280660.10563716910754546No Hit
TTAAACCCATTAACATGCAGGATCCTCATCTGCTTCACAATGGTGCTTTT278750.10491826725834924No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC277870.10458704546395517No Hit
CCATCAGGAGTCTGCCAGGTGACTTCACACGTGTAGTGGCTCCGGTCATC274320.10325086663429726No Hit
ACTTGCACATGGACAACGCCGCCTTCTTCTCCCGGAGCCAGCTCGAGTTG274180.1031981722579164No Hit
AGGTTGTATCTGGCGCCCTTCCCATCCTTATAGGTGCTGGTCACGATCCT271330.10212546531016287No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG269490.10143291064915708No Hit
TGCAGCATAGATCAGGCGCTTAGGGGCTTTCCCTGGTTTCTGCTGATACC267400.10064625888747117No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)