FastQCFastQC Report
Thu 9 May 2024
EGAF00006139921

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139921
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84140008
Total Bases4.2 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT9343841.1105109474199242No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA7285150.8658366184134425No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT7202140.8559709193276995No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT4901690.5825635291120962No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG3701020.4398644697062543No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG3579390.4254088019578035No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC3457250.4108925209515074No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA3434340.40816967832948153No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG3350830.3982445544811453No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA3167520.3764582480191825No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC2955760.3512906725656598No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG2917030.3466876304551813No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT2906610.3454492184027365No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT2846780.3383384513108199No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG2776760.3300166075572515No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT2579020.3065153024468455No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC2419870.2876004005133919No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC2416380.28718561567049056No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG2247550.2671202503332303No Hit
GTGGGCATCCGGGCTAAGGCTTTACTTGGCGGCAGTCTCATCGGATTTTG2122310.25223553579885566No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG2113600.25120035643448No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG2049980.24363914964210606No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC1930690.2294615897825919No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC1885550.224096722215667No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC1708710.20307937218166178No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC1600470.19021509957545998No Hit
GGGTAACCGAAATTGGAGAGCATGTCGGTCCAGAGACACTCGTTCTTGGA1571550.1867779713070624No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA1500770.17836580191435208No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA1477640.17561681239678514No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC1461240.17366768018372425No Hit
TAGACACGGACTCGGTAGTTGGACTTGGTCTCCTTTCCATTCCTGTTCAT1448170.17211431688953488No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC1433030.17031493507820916No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC1403830.1668445289427593No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT1402520.1666888360647648No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG1397600.16610409640084653No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT1374230.16332658299723482No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC1347930.1602008404848262No Hit
GGCGGCTGCTGCTAGGGAGTCGACTTCTCTCCGGTGGCGACTCCGCTTTT1318690.15672568036836887No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG1295380.15395529793626833No Hit
GAGTGAGCAGACACTCGGGAGGTGTCTTGAGTTTATCATCCGCTGAGGGT1276530.15171498438650016No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT1270550.1510042642258841No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG1268520.1507629996897552No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1248920.14843354899609706No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA1225270.14562275772543307No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1216480.1445780703990425No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA1210660.14388636616245626No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC1203060.14298310977103781No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG1188320.14123126776978676No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1183630.14067386349666142No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1144710.13604823997639745No Hit
GCTTGTGGGAGATTTTCATCTTCTTGGTACAGCCGCTGTAGATCTCTTCA1140370.13553243303708742No Hit
ACGTCATCCGTTGCCAGGTCTTCCTGCATGAGCTCACCAGGAACCTCTTC1122380.13339433007897977No Hit
GCAAGGAGATGGCAACAGCCTTCTTCTGCATCTTCCCCATCCAGATCTTC1116740.13272401875692716No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1113800.1323746011528784No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC1098830.13059542376083444No Hit
TGGCACCAAACACCACTCCAGTCTTCTTAATGTCTCTCCAGTACAGGAGG1090590.12961610367329654No Hit
CTGTTGAAGAGGTCGGCGAAGAGCTCGTCGTGGCACAGCTCCAGGGTGGG1084000.12883288530231657No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC1069830.12714878753042191No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG1069740.1271380910731551No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1046130.12433205378349857No Hit
GCAGCAGAAAGCCCGTCATCTCGGCGCTTTCCCTTAACCGAGTCGCTCTG1031640.12260992416354417No Hit
TACCAGAGCTGCTCCTTTTACTGCTTCCACCAGCCAGGGCTCTAGAGGTC999370.11877464998577134No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC998650.11868907832763695No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG959100.1139885796065054No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG958860.1139600557204606No Hit
TGTCAATTTCTGGGTGGATCCAAGTGCCCTTGTAATCTGGGTTGTCGATC957210.11376395400390264No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC953570.11333134173222327No Hit
GCTTCCTGCGTCTCAAAGTGTACAAATCCATAGCCCTTGGAACCATTTTC952270.11317683734948066No Hit
ACCAAAGATCACCCGGCCTACATCTTCATCTCCAATTCGTAGGTCAAAAT951840.1131257320536504No Hit
GAGGATTGGGGTTCAGAGCACTTCTGGGGTGTCAGCTCCTCCTTGTCCCC947330.11258972069505865No Hit
AGCCTCTGGATGGCCCGGTTCATCTCTGAAATCTCATTCCGGGTATTCCG926190.1100772417326131No Hit
CCAACTTCGGATTCCGAGTTCAGCTCCAACTGAAGGTCCCTGATGTAGTC905290.10759328665621234No Hit
GGCGTTGCCGCCTTTGCCACAAGATGACCCCAATGAGCAGGGCGGCTGTC900500.10702399743056834No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG892310.10605061981928977No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA880650.10466483435561356No Hit
TTCGAGGCAAGGAGAAGCAGGAGTTCCCGATCAAGAGGAAAACACGCAGC877300.10426668844623832No Hit
TTCCCCGCGGCCTTCGCTTTTAGCTCCTCGAGTTTCTTCTGCTCCTCTTT873210.10378059388822497No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)