FastQCFastQC Report
Wed 8 May 2024
EGAF00006139923

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139923
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31648671
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length52
%GC57

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT5783241.8273247555955825No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA4410331.393527709267792No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC2958320.9347375123587338No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG2422000.7652770000989931No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC2343520.7404797503187417No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG2248110.7103331447946108No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC1986160.627565056365242No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG1903020.6012953908870297No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC1681610.5313366870918529No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG1579000.49891510452366233No Hit
GCTTCTGAGGCTGTCTCCTTGGCATGTGATCCTGACTGTCTGTCCCAAGG1560540.4930823161579202No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG1547270.48888940707810447No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC1502260.47466764086239194No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC1382870.4369440979054065No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC1377050.4351051581281249No Hit
TCATTGCACACTCCTGGGAGCTCCTTCAGCTTTGTCTCTGATTCCCTGGT1281700.4049775107460279No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC1250260.3950434443203002No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA1244670.39327717742081486No Hit
GCTTTGGCCATGCCGGTTCCCTGCTCTCTGTCGGCTCCGCTCTGAGATTG1238610.39136240507539793No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG1231310.38905583112794845No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1169950.3696679712080169No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC1158120.3659300575370132No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA1089130.34413135388844607No Hit
TTGCTGTTCTGGTCATGGGCCGAGTTGTAGTTGGTCACGACGCGGGTAGG1079520.34109489147269406No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1044370.32998857993120784No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG1012290.319852293323786No Hit
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT1003740.31715075808396503No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG971480.3069575970504417No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC931240.2942430031264188No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG924400.29208177493456206No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT847850.26789434538973217No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT830900.2625386702651748No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT785400.24816207922285266No Hit
TTCTCCTCGGAGCAGGGCGGGCAGTGGATGGCTTCGTCGCCCAGGCTCGG775070.2448981191026947No Hit
AGGAGCCTGCGCGGGACCCCAGCATCCTGAGGCTGCCCAGGGTCGTCGGG771860.24388385850388472No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG763420.24121707985779248No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT761160.2405029898411848No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT746150.23576029464238796No Hit
ATGACCTCACAGCTCAAGTACACCTGGGCACCAGTGACATTCCAGATGTC726940.22969052950122296No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC690730.21824929078380573No Hit
ACACTCATCTGGACAACCCCTTCTTCCCAGCCTCGGATCACCTCCTGCTT664850.21007201218654648No Hit
ACGCTGTGGCAGCTCTTGCCCTGCTCCACGCAGACGTCCAGCAGCGCCCC663590.209673891203836No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC659290.2083152243580781No Hit
TCGGCCACGACCCCTCGTACCACCTCGTCCACCAGCTGCAGCACCTCTTC603850.1907979011188179No Hit
TCTTTGCCACTCCTGCCCCATTCATTGGCAGCCTGATCAGCCAGCGAGTC585750.18507886160527878No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT572480.18088595252546308No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC571300.1805131090654644No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG565810.17877843906936883No Hit
CAGCACCTCTCCCTGGCCAGCACTTCTGGTCTCCACAGTGAACTCTGCCC565150.17856989950699667No Hit
CAAGCTGGGCCCGCGACTCGAAGGTGACTGGGATGGTGATCTCGTTGGAC554070.1750689626114158No Hit
CTGAGGAGCTGGTTCTGGTGGAACTTGGAGCTGGGCAGGGTGACTGAGTG546020.17252541188854342No Hit
TGCACCTGGCCCCGAAGCTTGTTGTGCAGCATGAGGATCTCCTCCTTGTC545870.17247801653345887No Hit
AGGAGGACCCGGGAGCCCATCTCTTCCACTTGGTCCTGGCAGAGATGTAC543790.17182080094295268No Hit
GTATGTCACTGCAGTCGGCGGCAGTGGCTGGGCGGATCACGAATTTAGCC529920.1674383104427987No Hit
GGGTCCACCTTCCCAGGGAAACCATGGCTTCAGCAACCAGTTTGTTCTCC529750.16738459570703618No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT521140.16466410232518136No Hit
GTCACCTCGGTCCCTTCTGAGACCTCTGGCTTCGTCAGAATCACGTTGGG516040.1630526602523057No Hit
CATGCCTGGAAGACCTGTCCACACTGGTAGCCAACCATGAGGCTGTACTC504380.15936846131706447No Hit
GTCTTCACACCGTTCTCTGCCATCTCACACACAGACTTCAGGTAGGGATA494120.1561266190292793No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG488120.15423080482589616No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA484530.15309647599420526No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC482660.15250561390081752No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA482110.15233183093217406No Hit
GCCGCGTCCTCCTTGCCCAGAAGGTAGGCCTTCACGGTGAGCGACGCCAT471090.14884985217862703No Hit
GGTCATACTCCTCCACCTGGCCGCTGGTCCACACCTTCACGCGGTCTGTG465750.14716257753761602No Hit
GCTTCTTCGCCGCTCCTGCTGGCTTCTTGGCCTTGGCCGCGCCTGCCTTT456420.14421458645135524No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC454550.1436237243579675No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG448540.1417247504642454No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT442830.13992056728069244No Hit
GCGTCATCATCCACCACGGCCTGCTGTAGGGCCTGGTCATAGAACTGCTT442370.13977522152509975No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG437110.13811322440680052No Hit
CCAGCACGGCGTCCGACTCCTCGGTGATCTCCAGCAGCGCGTGCACGTCG431250.136261645868163No Hit
ATTCCTCGGGCGGAGTCCCACACGTGTTGGTGGCCACCACAGTCACGTTG423610.13384764244918848No Hit
ACCTCCTGCCAGATCTCCGTGACCTTGGCGTACATGTCTCGGATCCACTC421590.13320938500071614No Hit
ACCTTGCTGGGCTCCTCGGCGGCCTTGGTCTCGTCGCTGGCGGGCGGCTT413270.13058052263869152No Hit
GCCCAGTGCCAGCTGGTAGTGGTCTACCTCACCGAGGAGGCGGAAGGTCG406000.12828342776225896No Hit
ACTTGCACATGGACAACGCCGCCTTCTTCTCCCGGAGCCAGCTCGAGTTG403700.12755669898429542No Hit
GAAGGTCCTCTCGTACCTCCCCGCTGTCCTGGAGCAGTGATAGGTACCCA403450.12747770672582112No Hit
GCACACTCATACTCATCACAGCACTGGGTCTTCCGAAGGGTGGGCAAACG397800.125692481684302No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT396080.12514901494599884No Hit
CATCCAGGATTCGGGTCACCTCGCTCTGCTGGGACTTGATTTCGCGGGAG393310.12427378072210361No Hit
GCCTCGTCAGATCCGTCTGGGCAGTCCCTCTCACCGTCGCACCGCCAGCC392800.12411263651481606No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG389470.12306045963193843No Hit
CGATCTTGACTTGCTCGCGGTAGCTCTTTTCGTTGAGGCAAACCCCGCGG380960.12037156315347333No Hit
CGCGCCGGATCTTGTACTCGTCGCTGTGCTTGTCCACGGTCTTCTTGGCC378770.11967959096923848No Hit
ACGCCGATGCCCCATTTTCTGTCTTCTGCCGTTTGGGATCCGCTTCATCC377350.11923091494110448No Hit
CGGGCCTCGTTCACACGCTCTCTGTCATTGCTGTCCACCACGAAGATCAG373950.11815662022585405No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG370260.1169906944907734No Hit
AGAGGAAGCCATCGCTGCGGCCGGTATCAGTGCAGCTGTTGTACTCCTTG367140.11600487110501416No Hit
AGGTCCCTGTGCACGATGGGCATCTTGGGCCTCCCACATGGAGTGTGATC366740.1158784834914553No Hit
CTCCTCCGAGCGCTCCAGCTCTCCTTCCAGGATCACCAGCTTCCTGGCCA366340.11575209587789642No Hit
AACCCCACTCTGCTTCAGGTTACTTGCTGGCCCATCCACACGGTCAGATG363600.11488634072501812No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC363160.11474731435010335No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA362550.11455457323942607No Hit
CGGCCAGGCACACGAAGTGCGGCATCACCGCCTGGTAACTGGAGCAGATG361600.11425440265722374No Hit
TTCCTGCTTCATGCGGGCCAGCTCTGTGCGGACATGCTCGTCGCTGCGCA358020.11312323351587181No Hit
GAATCAATGTCCAGGCGGCACATGTGCTCCAGGAATGTGTCAGCCATGGC356360.11259872491960246No Hit
TGGGAGGCTCAGGAGCTGGTTGTTTAGCTTCTCGGGCACGACGCTGAGGA346650.10953066560046075No Hit
AGCGGAGGTCCTCCACGCCGTCATTCACCATGTCCACCAGGGCTGCCTCC345240.10908514926266573No Hit
TCGATCATAGTCTTCCTGCCCTGCTGTGTCCCACAGAGCCAGCTCCACCT343230.10845005150453237No Hit
GAAGGACGCCGCCCTGAGCAATGGTCACCCGGCCTAGCAGTTTGTTGAGC342400.1081877972063977No Hit
AGCTCCTTGGTCAGGCTCTGCACCTGTTCCTCCAGCTGCTGCACCTTCTC337030.10649104349436979No Hit
ACGTCATCCGTTGCCAGGTCTTCCTGCATGAGCTCACCAGGAACCTCTTC336230.10623826826725205No Hit
AGCGCTGCGCCATCGCCCTGGATCTTCCTCACTTGCTCTAAGCACTTGAG328120.10367575940234584No Hit
CCCGCACCGTGGAATGCGGCCATCTTGTCATCTGCCACCACCAGTGTCTC326730.10323656244522873No Hit
GCTGGTGGCCCCAGACCTGCCCATTGGCTTTGTGTCCTTGGCGCCGGTGG325050.10270573446828146No Hit
AGCATCTGTTCCAGCTGCTGCATCTTCTGCCTGGTCACTGACTGGTTGTT324190.1024340010991299No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA322450.10188421498014877No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC319230.10086679469099984No Hit

[OK]Adapter Content

Adapter graph