FastQCFastQC Report
Wed 8 May 2024
EGAF00006139927

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139927
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29828308
Total Bases2.2 Gbp
Sequences flagged as poor quality0
Sequence length75
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGGCCTCTCTGGGATAGAAGTTATTCAGCAGGCACACAACAGAGGCAGTT5491711.8411067768242169No Hit
TGGGCTCAACTTTCTTGTCCACCTTGGTGTTGCTGGGCTTGTGATTCACG4784851.6041305460571211No Hit
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT4120931.3815500362943818No Hit
GTGTAGGAGGCCTGCAGGTACAAATTGACCAGGCTGTTGACGGCTGCCTC4009551.3442096682118208No Hit
AGGGACGCCATCTCTGCCAGGTTCTCCAGCAGCTCCTCTGGGCCCTGCAG3634301.2184063541250816No Hit
GGCAGCTGTAGCTTTTGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGG2901080.9725928805616464No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT2565980.8602499343911831No Hit
TGTGGGACTTCCACTGCTCAGGCGTCAGGCTCAGATAGCTGCTGGCCGCG2537710.8507723602693119No Hit
GTGGGGAACGAGCGCCGGGTTCCGTCCAAGCACTGTTGAAGCAGGAAACC2258720.7572404039813455No Hit
CCAGGCTCCCCAGGGCGACCAGCATCTCCATTAGGTCCTCGGACTCCAGC2219920.7442326262689791No Hit
ATCTTCATGAGGTAGTCAGTCAGGTCCCGGCCAGCCAGGTCCAGACGCAG2118600.7102648933355523No Hit
TGTGACTCGTGCAGCCATCGACAGTGACGCTGTAGGTGAAGCGGCTGTTG1890270.6337168035142993No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA1865520.6254193164426223No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG1726470.5788025254399277No Hit
GGCTCCATCCTCTGGCTCGCGGCGTCGCTGTCGAACCGCACGAACTGCGT1726340.5787589426795512No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1317120.44156711805443344No Hit
TGGGGTAGGTGGAGTTGAGGGAACTGGGCAGGGCACAGTCACATCCTGGC1237930.4150185119450959No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1210170.4057119163447019No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1197910.4016017267891963No Hit
CCAAAGGTGGATAGTCTGAGAAGCTCTCAACACACATGGGCTTGCCAGGA970750.3254458818113317No Hit
ATGGCCTGCACCTCGCCGCGGTACTGCACCAGGCGGCCGCACACGTCCTC959860.3217949874997938No Hit
AAGCGAGGCGCACGAGTACGAGCGAAGTCTGGTCTGCGCAGTGGCCACCA951980.319153201716973No Hit
GATCTTCGGAAGAACGGATGATCCTGGAAGTAATCCGGGCACACTTACAT948570.3180099923870975No Hit
ACATGCTCTGGCCGAGCCAGTCGCTCTCTTTGATGGTCAGTGTGCTGGTC932610.3126593704208767No Hit
TCCATGCCCAGTAGTGAGTTCTTGTCGGTGGCCTGCAGGAAGACGTTCTC931310.31222354281711184No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG896600.3005869457965903No Hit
CATTCAGGATCGCAGACCAGCTCATCAGCGAGGCAGCCACCGAGTTGGCC896470.3005433630362138No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC896310.3004897227157504No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG894390.29984603887019No Hit
AGCCACAGGAGCCCTGGTCTCTGATCTCTTTGATGGTGGGACACTGTGGC886640.2972478358477457No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC864170.2897147233426717No Hit
GTCACCAAGTGGGGTTTTGAGCTCAACTCTCTTGTCCACCTTGGTGTTGC857290.2874081895627469No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG857230.28738807444257314No Hit
GGGTTCTCCCTTCTGCCCTTTGGGTCCTCGGATCCCGGGAATGGCTGGGA830700.2784938388057412No Hit
GATGGTGACTCTGTCTCCTACAGATGCAGACAGGGAGGATGGAGACTGGG830310.2783630905246117No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC829620.27813176664261346No Hit
GGTGAATCCAGAGGCTGCACAGGAGAGTCTCAGGGACCTCCCAGGCTGGA812790.2724894754338731No Hit
CTTCATGGCTGTGAAATTCATGGCTGTGGAATTCACGGCTGACTTTGGAA768030.2574835957842463No Hit
CAGCCCATCATTTCCAGGAGGACATGGCATTTCTCCAGGTGGACCCATGG714620.23957778630956875No Hit
GGTCAATCCGGAGAGTCCAGTTGCTCCCAGTGACTGCAGAGTAGCCACAA712010.23870277858201008No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA693780.23259113456921524No Hit
CACTCTGGCTGGACTTGCAGTTGATGGTGGCCCTCTCGCCCAGAGACACA681190.22837031185275408No Hit
CTTCGCACGCAGCACCAGAGGCTGCCATCAAGATGAGGGTGAGGGCCAGA679870.2279277792089313No Hit
GCACTGGGATGACGATGAGCAGAATGGTCATGAGGATGCCCAGAATCAGG677620.22717346220241524No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG673320.22573187858996227No Hit
GAGAGTCTCAGGGATCCCCCAGGCTGTACCAAGCCTCCCCCAGACTCCAC669060.22430370505762515No Hit
ACTGGGCCTAGGGAGGGCTCGAAGTCGCGGTCATCAGGAACTTCTGGGCC646970.2168979883136516No Hit
GAGGAGTGGGTGTCGCTGTTGAAGTCAGAGGAGACCACCTGGTGCTCAGT632960.21220110775307804No Hit
TGCCACAGGTGGTGCTGGGCACGGTGGGCACTCGACACAACATTTGCGCT624870.20948891904964909No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT610660.20472498808849632No Hit
TTTGTGAAACTTGTTTCCTGCAGGTGATGGTTCATGACAATATCGACACC581160.19483505400306314No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC575540.19295093774678737No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT572220.19183790109717253No Hit
CAGGCCACTGTCACAGCTCCCGGGTAGAAGTCACTGATCAGACACACTAG550580.1845830477545022No Hit
AAAGCACATGCACCAACACCAGGCTCTTCTTTGGCCAACTGGTACCACAT538630.18057678631989452No Hit
ACCTGCTCTTGTCCACGGTGAGCCTGCTGTAGAGGAAGAAGGAGCCGTCG537210.18010072847578212No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG517950.17364377490000438No Hit
GGTTTGCACCGCCCCTGTGTCATATTCCGGCGCCTCATCATTTGGTTACA517070.17334875313745587No Hit
TCCGGTGGTATCTGGGAGCCAGAGTAGCAGGAGGAAGAGAAGCTGTGCTG516740.17323811997650018No Hit
GCTTTCAGGGCAGTTACATTCTGGTGCACAGTTTGGTGATGATTGCCCAT509150.17069355727451924No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG492770.16520212946708207No Hit
GTGATGTTGGCGGTTAGTGGGGTCTTCAACTCGGGGTGGGCAGCAGTGCA475250.15932851437634343No Hit
TAGTTGGAGCGCTTTGTCATGATTTCCAGGTTGGCTTTGTCCACAGCTAT469050.15724995195838798No Hit
AAACCTTCACTGAGGCCCCAGGCTTCTTCACCTCAGCCCCAGACTGTACC465260.155979346867412No Hit
GTGCACAGGAGCAGAGGGCCATGGTGCAGACGAGGACAAGGAGGGCAGCT454800.15247261091711942No Hit
TGGTGCAGACCACACCAGCGTCTCTCTCGTGCCTGCAGTTGCTCTTCAGC447880.1501526670570788No Hit
GTGCTTCATAGTAGAGGGTCACATTGACAAGCGGTGCATTGGACTTCTTC438620.14704823351026147No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC429660.14404437556431293No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC428260.14357502276025846No Hit
CCTCAGCCTGGAGCCCAGAGACGGTCAGGGAGGCCGTGTTGCCAGACTTG422100.14150987042241886No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA413470.13861664563742604No Hit
CAGCAGGAGCCCCAGGAGCTGAGCGGGGACCATCATGTCCATGCTGTGTC407670.136672184020629No Hit
TCCATGACCCCGAAGCCACTGACATAGCCCATCAAGCCCAGGTCGTAGAA406140.13615924845619806No Hit
CATGCTGGAGATTGTCCTGAGTCCTGTCTTCTCTACCCACAGCTGAAGCT403080.13513337732733616No Hit
ACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCCACCCAT396730.1330045271089463No Hit
GTGGTCTCCACTCCCACCTTGACGGGGCTGCCATCTGCCTTCCAGGCCAC393350.1318713753391577No Hit
GGCTCCACCTCAAGGGTGATGGTCTTGCCGGTAAGGGTTTTCACGAAGAT392530.13159646869678293No Hit
GGTGCTCCGCCTCAGAGGCATCATTTGACTTTTGCTCGGAGATCTGAGCC388190.1301414750042141No Hit
AGGAGCTGCAGAGGGTCAGGATGCTCAGGCAGAAGTCCAGCAGCTGCAGC387500.12991015112221585No Hit
TCCCACCGGTGGACTTACGAGCAGTCTGCTTGGTTCGGGCCATTTTCTTT357270.11977548307466852No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG344770.11558483303846802No Hit
TGCAAATCCAGCAAGAAGTCCCAGTCTTTTCTGTTCAGTTTCATGGCTAT340310.11408960910555169No Hit
CCTGCACACCACACCCACAGTGGTTTCAGACATGCTACTCTTGCCAACAG338390.11344592525999128No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG335910.1126145002928091No Hit
CAGCACCAGTTCAAGAAGAGGGTGTTGAACAGGGACCAGACGACATGGTC334210.11204457188788582No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC327950.10994589434975661No Hit
CTGACTCCCCAAAGACATGCCTTCTCTCTGAGTGCCCCAGGACCACCCAC327610.10983190866877196No Hit
CCATCAGGAGTCTGCCAGGTGACTTCACACGTGTAGTGGCTCCGGTCATC320660.10750190724864447No Hit
ACTTGCACATGGACAACGCCGCCTTCTTCTCCCGGAGCCAGCTCGAGTTG315950.10592287031500412No Hit
GGCGTTGTCTCTGGAGATGGTGAATCGGCCCTTCACTGAGTCTGCGTAGT314630.10548033767118134No Hit
ACCAAAGATCACCCGGCCTACATCTTCATCTCCAATTCGTAGGTCAAAAT311990.10459527238353579No Hit
AGGTTGTATCTGGCGCCCTTCCCATCCTTATAGGTGCTGGTCACGATCCT307050.10293912748922936No Hit
AGCTCCAGGATCTCGGCGGTCAGGTACTCGAGGACCGCCGCCATGTAGAC305920.10256029272595685No Hit
AGCCTCGATGCACCCTGAGCCAGGTGTTCTCCAGGGTGTGCTGACTGCAG305690.10248318476529074No Hit
TTAAACCCATTAACATGCAGGATCCTCATCTGCTTCACAATGGTGCTTTT304190.10198030676094667No Hit
AAGCGCACGGACTCCTCCTGGTTATAGAAGTATCTGTCCAGGAACCGCAC303470.10173892531886153No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)