FastQCFastQC Report
Sun 12 May 2024
EGAF00006139931

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006139931
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102872266
Total Bases5.1 Gbp
Sequences flagged as poor quality0
Sequence length50
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGACCTCAAGTGATCCGCCCGCCTCGGCCTCCCAAAGTGCTGGGATTGT28673812.7873217063187856No Hit
TTGCATCCCACCTCTGAGACGGAGCACCAGGTGCAGGGTGGACTCTTTCT14923581.4506903153081123No Hit
GAAGCTCAGCTGCTTGTCTGCATTTGTGTCCAGGTCCTCCATGATGTGTT13798131.341287650842648No Hit
CTTGTCACAGTGCAGCTCACTCAGTGTGGCAAAGGTGCCCTTGAGGTTGT12976631.2614313366053393No Hit
GGCGAGCTGAAGAGGCGGCTAGAGTCGGGACAGGTTGCACGCGGGTTCGC11839641.1509068926312949No Hit
GCAATGAGAAACTCCTCGCTGCGGTTGTGGGACCTGTGGAAGTATCCGCA10112720.983036574697402No Hit
CTGCCCACTCAGACTTTATTCAAAGACCAGGAAGGGCCGGTGCAAGGAGG9420830.9157793802267368No Hit
CTGAGGACACTCGGTCTCTAGCAATTTCTTCAGGTCATCCCTGTAGACGG8533620.8295355329297402No Hit
CCAGTAATCTGCCCAGGGCCTCACCACCAACTGCATCCACGTTCACTTTG7393110.7186689170432No Hit
TCCGGTCCAAGTCTTCCATCAGCCTTGCAATTTCAGCATCCTGCAGCTTC5143210.49996079604195753No Hit
GAATAATTCTGACGAATCTGGGAGCTCATGGTTGGTTGGCAAGAAGGAGC4940840.4802888273113377No Hit
ATGTCTCGATCCCACTTAACTATCTTGGGCTGTGACAAAGTCACATGGTT4879390.4743154000321136No Hit
TCACACCATCCTCACGGTAGTCCAATAGAGCAACCATGCCATCTGGATTC4833050.46981078457044967No Hit
AGCTGCATGATCTGCGCGTTGATGTGAGGTTCCAGGGCGCCGTAGTCGTA4698640.45674506674131204No Hit
ATCTTGTTTTCTGCGCAAGTTAGGTTTTGTCAAGAAAGGGTGTAACGCAA4640350.4510788165198966No Hit
GGTGTCTGGGGCGCCCGACGATGGAAGGAAACACGGCTCGGGGAGCGTCG4446770.43226130549121955No Hit
ACATAGGGACAGTTGTCCAGATTGTTCTGGTGGCCATCTTCATCAATATC4086460.39723631634594303No Hit
TAGGAAATTTCACTCCGCTTCTCCTGCTCAATGGTCTCTTTGGTCGGCAG3995570.38840108761675374No Hit
ATGCTATGAAGCCCAGGCAGCAGGTGTTCATGAAGAGGGTGTTGAACAGG3700860.35975293865889957No Hit
ACTCGTGAAGTCAGCCTGAAACTGTCTGAACATGTCGGTCATTCCCAGGT3181380.3092553633454521No Hit
GGAAACGGAACACTGGATCCGCGAGAAGAGCTCGGTCCTTCCGGACGCCG2835380.2756214196739868No Hit
GGGTCCACCTTCCCAGGGAAACCATGGCTTCAGCAACCAGTTTGTTCTCC2668480.2593974162093406No Hit
TGTAGGAGTCGGTGGCCAGGAGGATGTTGAACTCGGCTTCTGCCGCAGGA2535760.24649597978137275No Hit
TGCCCAGCACGTACAGCTTCACCATCTTCCCCTTGCGCTTCTGGGCCATC2473860.24047880893378978No Hit
GCCCATTCACGTAAAAACTTGACCGGTCTTTGCCAACCAGGACACCCACC2400080.23330680788153338No Hit
TCACACACAGACTTCAGGTAGGGATACTGGTCCTTTGTACTGAGATAGGC2269090.22057354117192285No Hit
CCCACGGCTATGGGGAAATTAGCCCGAGGCTTAGCTTTCATTATCACTGT2229050.21668133566728276No Hit
GGAGCAGGTTTCCTCGATTATCCTTCTTGCTCCAGGTGTCTCCAATTCTA2101140.2042474693811061No Hit
TGATCTATCCATAGGCCTGGAAGATCAGCACTGGGATGACGATGAGCAGA2089400.20310624828658871No Hit
TCCTCAGAAAGTGGGAAGATACGATTCAAGTCCTGGATTCTCGTCTTTGG2086040.2027796296428427No Hit
ACCGCCTTCTTGCGCTTCTTCACCTCCTCTGGCGTTGAAGACTTACGCAC2083170.20250064288464298No Hit
TTCTTTGATGTGAAAGGGGCAGCACAGTCATTTAAACTTGATCCAACCTC2073160.20152759150848296No Hit
AGCAAAGTCCTTGAGTCCCAACAGTCCACCTCAAAGACAAACATACTAAA2033290.19765191135188953No Hit
CAGCCCCTCATGCTGTACATGGCATGTGTATCTCTGCTCTTCTCCAGAAG1971340.19162988010782225No Hit
GGAGGCAGTTGCAGGACTTCTCGTTCACGGCCTTGGCGATCCTCTTTGGG1796670.17465057102951342No Hit
GTGTGACCACACTTCTTCTTGCGGCAGTTGACAGCACGAGGGTGAAGGCG1795860.1745718326064675No Hit
CACAGCACTTAAAATCTGCCTGCATCCTGTCCAGGATCGAAGCAGTGTGG1739620.1691048586409091No Hit
GATGCCGACCACCGTGCCCACGTCCTCCTTCTTGTCCTCCTCCTCGGCCC1688630.16414822630620385No Hit
TATTCACGAAGGTGACGAGCCATTTCCTCCTTCATATTCTGAATCTCATC1672480.16257831824176985No Hit
TCATCCCTCAGCCAGAGATTAGTCTACTTCTTCCATGCGTGATGTGTCGT1672090.16254040714919218No Hit
TTGTCCTCCTTTTCAGAATACTTTTCAGATGCAGCCTCCAGAGATTTCAG1648530.16025018832578258No Hit
GTCCTTCGACATAATCCTCATAGGTGCCCTGGTCCTTGTTCTTGGCCACT1578750.15346701899227144No Hit
GCTTCCTGCGTCTCAAAGTGTACAAATCCATAGCCCTTGGAACCATTTTC1495420.14536668221151072No Hit
ATTGATCTTCTCGGTCTTGCATCATACTCCTGTAGGCGGTGTCTGTGATG1494710.14529766458143345No Hit
GGCATGATCTCGGCTCACTGCAACCTCCGCCTCCTGAGGTCAAGTGATTC1479090.1437792767197332No Hit
GGAGCTGAGCGAGGCGCGGGAGGCTGTGGCTGGGAGCGGCGCTGAGAGCT1475230.143404054111144No Hit
AACCGCAGTACTTGGGCCGGTATTTCTTCACACTCAAACATCCAGCGTAA1469870.14288301960802535No Hit
TTCGAGGCAAGGAGAAGCAGGAGTTCCCGATCAAGAGGAAAACACGCAGC1469000.14279844870919825No Hit
CTGGCACCAGTAGCTTGGGCCCCATATACACTTCTCAGTTCCCAACAAGG1428830.13889360617369895No Hit
AAGACAGCCAGGACGAGCAGCAGCCCGAACAGGACAGACCCTCCTTGGAT1393730.13548160784170926No Hit
CATTCCACTCCATACAGGCACACTGGAATCTCCATGTTCCCCAAGGACCC1382590.13439871150500368No Hit
TCTTCAGCACACAACGCCACTTGGCGAAGTCAGCTCCGTCCTTCTTGTAC1376410.13379796649954226No Hit
TTGCCATCTCTACACTGGTCCAGGTCCTTCATTATTTTGTCCACAGCCAG1337590.13002435466911946No Hit
CGTGTGTTGTAACCACTCTCCCATTTGGCCAAACACATCCAGTTTGCTAG1317030.1280257596347688No Hit
GTCAGTTTGTCCAGCCGGCCCTGCTGCTGGTACAGGAAGTAGGCGGTGGT1307400.1270896472718896No Hit
GGCTGTGGATACACTGGAGAGGGCTGGACATGGTCCCACACACCCCGAGG1266550.12311870334420358No Hit
TGCTACATCTACACTTGGTTGGCTTAATCAACCTCTTCAATGGTGGGCCC1264120.12288248807506583No Hit
GAGTGAGCAGACACTCGGGAGGTGTCTTGAGTTTATCATCCGCTGAGGGT1241590.12069239341923313No Hit
ACTCCTCGAACACGTGGCCCCGCTTCCTGTTCAAAACCCCGTAGATGCCA1223420.11892612533683276No Hit
TTGTTCTCATCCAGCTCGCACACCTTGCCGTGTTTGCAGTGGTGGTTCTG1208940.11751855451497492No Hit
CCAAAAGCGCCAATCCTGTTTGCCATATCTCTGCCTTCTTCAGGTTTCAC1198430.11649689917397173No Hit
ACTTGGACAGTGAGGAACATTACCTCCTCATTGGATGAAGACTTTGGGAC1185590.11524874935679943No Hit
AGAGCCAGAACGAAACTCCCTCATAATCACGTCTCGTTCCTTTTGGTCCA1183210.11501739448414601No Hit
CAGGAACAGGCCCCGAAGAAGCGCTCCCTGCTCAGGACGCGCCACCACAT1175340.11425236807751468No Hit
ACCAGCTTCTGCTGCATCAGGTTGTCGAAGACGGGCAGCACGTTGTTGAC1171810.113909224085722No Hit
CTCTTGCTGGGGCTGGTGGTCCAGGGGTCTTACTCCTTGGAGGCCATGTG1162500.11300421826034239No Hit
CTGCCTGAGACGCCCCGCGGTTGGTGCCCATCTGTAACCCGATCACGTTC1149460.11173662685723283No Hit
CACGAAGCCACAAAGCTACTTGGGTTCCTCCTTCTTCTCGTTTGCCTTTT1140420.11085786717286852No Hit
TGCCTCTGCTTCAACAATGTGCAACTCATCCTTTGCACCAGCCCCTAAAC1109550.10785705838345196No Hit
CCTGCACACCACACCCACAGTGGTTTCAGACATGCTACTCTTGCCAACAG1081410.10512162724207903No Hit
AGGAGCTGCAGAGGGTCAGGATGCTCAGGCAGAAGTCCAGCAGCTGCAGC1074760.10447519450966503No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1059790.10301999180226087No Hit
ACAGCAAGGAGTAGTTGCTCTAAATTGCCAGAAGTCTCGCGGTCAATGGT1059320.1029743040753083No Hit
GCAGCAGAAAGCCCGTCATCTCGGCGCTTTCCCTTAACCGAGTCGCTCTG1048330.10190598892805569No Hit
ACCATCTGATTTCTCATAGCTGGTTATAGCTGCCTTTCGCACTTGGATCT1046770.10175434455774504No Hit
AATGGCCAATTGAAACAAACAGTTCTGAGACCGTTCTTCCACCACTGATT1038330.10093390963119254No Hit
CGCGCCGGATCTTGTACTCGTCGCTGTGCTTGTCCACGGTCTTCTTGGCC1030740.10019610144487338No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)