FastQCFastQC Report
Sat 17 Dec 2022
EGAF00006141624

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141624
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23359173
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACACCAGTATCTCGTATGCCGT937360.4012813296087152TruSeq Adapter, Index 1 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACCAGTATCTCGTATGCCGTC755660.3234960415764719TruSeq Adapter, Index 1 (96% over 33bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACACCAGTATCGCGTATGCCGT339100.14516781052137417TruSeq Adapter, Index 1 (97% over 34bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACCAGTATCTCGTATGCCG286560.12267557588618398TruSeq Adapter, Index 1 (97% over 35bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC266430.11405797628195143No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACACCAGTATCGCGTATGCCGTC245330.10502512225068927TruSeq Adapter, Index 1 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)