FastQCFastQC Report
Sat 17 Dec 2022
EGAF00006141626

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141626
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16225563
Sequences flagged as poor quality0
Sequence length101
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1326610.8176049114597749No Hit
TAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA426620.2629307839734128No Hit
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC399540.2462410703406717No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA382880.2359733218502187No Hit
CAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT256360.15799759922044Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC255730.1576093230170195Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA197940.12199268524611441No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)