FastQCFastQC Report
Sat 17 Dec 2022
EGAF00006141644

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141644
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16858443
Sequences flagged as poor quality0
Sequence length101
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC517090.30672464829640556Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT511340.30331389440887274Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACAACAAGATCTCGTATGCCGT386980.22954670250390263TruSeq Adapter, Index 1 (97% over 34bp)
CAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA281970.1672574388987168Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACAACAAGATCTCGTATGCCGTC274350.16273744852949942TruSeq Adapter, Index 1 (96% over 33bp)
CAAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAG191810.1137768179422026Illumina Multiplexing PCR Primer 2.01 (100% over 30bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)