FastQCFastQC Report
Sat 17 Dec 2022
EGAF00006141651

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141651
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17217431
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAAGAGCACACGTCTGAACTCCAGTCACACACGCATCTCGTATGCCGT779880.45295956173717206TruSeq Adapter, Index 23 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACACGCATCTCGTATGCCGTC637650.37035141886150147TruSeq Adapter, Index 23 (96% over 33bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA526510.3058005575860882No Hit
CAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTC410080.23817722864694504Illumina Multiplexing PCR Primer 2.01 (100% over 32bp)
CAAAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGT400940.2328686550275706Illumina Multiplexing PCR Primer 2.01 (100% over 31bp)
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC326030.18936042200488562No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACACACGCATCGCGTATGCCGT261670.1519797001074086TruSeq Adapter, Index 23 (97% over 34bp)
TCGGAAGAGCACACGTCTGAACTCCAGTCACACACGCATCTCGTATGCCG244120.14178654179011957TruSeq Adapter, Index 23 (97% over 35bp)
CAAAAAAAAAAAAAAAAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA230850.13407923632741725Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
GGAAGAGCACACGTCTGAACTCCAGTCACACACGCATCGCGTATGCCGTC193810.112566154613891TruSeq Adapter, Index 23 (96% over 33bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)