FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141655

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141655
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29023513
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC5859282.018804546506827No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA1508070.5196028475257285No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACATTGGTATCTCGTATGCCGT1256260.43284215801167836TruSeq Adapter, Index 12 (97% over 34bp)
GGAAGAGCACACGTCTGAACTCCAGTCACATTGGTATCTCGTATGCCGTC930740.32068481854694847TruSeq Adapter, Index 12 (96% over 33bp)
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT758670.261398404803719No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC709850.24457756026984054No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT623550.2148430481175728No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAG605690.2086894167497918No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC476500.1641772310608988No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACATTGGTATCTCGTATGCCG443640.15285537625993106TruSeq Adapter, Index 12 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACATTGGTATCGCGTATGCCGT409580.14112006358430834TruSeq Adapter, Index 12 (97% over 34bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT406580.140086418897671No Hit
GCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT372400.1283097604345828No Hit
CGTGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC321670.11083082878354526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)