FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141661

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141661
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47497609
Sequences flagged as poor quality0
Sequence length101
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC6504731.3694857776946203No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA1782360.37525257324005507No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC830810.17491617314884209No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT764660.16098915631732114No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT737030.15517202139585595No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAG732230.15416144421080227No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACCTCATAATCTCGTATGCCGT594770.1252210400738277TruSeq Adapter, Index 1 (97% over 34bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT563920.11872597629072235No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC549220.11563108366149547No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACCTCATAATCTCGTATGCCGTC531300.11185826217062843RNA PCR Primer, Index 40 (97% over 34bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)