FastQCFastQC Report
Sat 17 Dec 2022
EGAF00006141664

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141664
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23438483
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC2852951.2172076153563351No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCGAGATCTCGTATGCCGT966540.4123731045221656TruSeq Adapter, Index 3 (97% over 34bp)
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA725350.30946968709536365No Hit
GGAAGAGCACACGTCTGAACTCCAGTCACTTCGAGATCTCGTATGCCGTC705600.30104337383951TruSeq Adapter, Index 3 (96% over 33bp)
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC358720.15304744765264885No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT346080.14765460716890252No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT321110.13700118732086886No Hit
TCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGAGATCTCGTATGCCG317420.13542685335053467TruSeq Adapter, Index 3 (97% over 35bp)
CGGAAGAGCACACGTCTGAACTCCAGTCACTTCGAGATCGCGTATGCCGT295740.12617710796385587TruSeq Adapter, Index 3 (97% over 34bp)
CGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAG270900.11557915245624045No Hit
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT237450.10130775101784531No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)