FastQCFastQC Report
Sat 17 Dec 2022
EGAF00006141669

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141669
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17353532
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC2117671.2203106549145153No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCA546280.31479470576940766No Hit
AGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC264760.1525683647570996No Hit
ACGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT250450.14432220483991384No Hit
TCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT241090.13892849017710054No Hit
CGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTCAG198570.11442627356782469No Hit
CGGAAGAGCACACGTCTGAACTCCAGTCACGGCTGCATCTCGTATGCCGT191460.11032912492972612TruSeq Adapter, Index 11 (98% over 50bp)
CCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGT178810.10303954261299658No Hit
TGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTAGTC173570.1000199844043276No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)