FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141801

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141801
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences164443952
Sequences flagged as poor quality0
Sequence length151
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA111778246.797345760700278No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCTTCCATCTCGTAT10994410.6685809886155011TruSeq Adapter, Index 11 (97% over 36bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7319850.4451273464894592No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5471240.33271153687671046No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5045220.3068048376750274No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4496660.2734463594015303No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCCTTCCATCGCGTAT4397480.2674151251242125TruSeq Adapter, Index 11 (97% over 36bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3310910.20133972455247243No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2805820.17062470014099393No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)