FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141807

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141807
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212549032
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44807902.1081206335486864No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGCAAGATCTCGTAT12776460.6011064778690688TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5601780.2635523647080171No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGCAAGATCGCGTAT4578350.21540206308725982TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4287260.20170687015878763No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3848330.18105610568012373No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3278530.15424817366375962No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)