FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141809

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141809
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185515556
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26465531.4265935736408002No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACATCATCTCGTAT5570100.30024975371876633TruSeq Adapter, Index 19 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4095240.22074914299909168No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3064400.1651829132862583No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2765320.14906135418638425No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2679090.14441322645740823No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACATCATCGCGTAT2213290.11930481991494017TruSeq Adapter, Index 19 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)