FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141811

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141811
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences220620096
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA53332662.417398095955864No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTAAGGATCTCGTAT8584240.38909601417270706TruSeq Adapter, Index 14 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6078280.2755089001502383No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4601740.20858208673791892No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4115020.18652063318837464No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3984700.18061364636519783No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTAAGGATCGCGTAT3047060.13811343822459402TruSeq Adapter, Index 14 (97% over 37bp)
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2595860.1176619921333005No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2444660.11080858200696278No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)