FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141813

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141813
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences222155096
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGTATATCTCGTAT18229740.8205861728240527TruSeq Adapter, Index 5 (97% over 37bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14043350.6321416997789688No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3899620.175535923785426No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGGTATATCGCGTAT3278650.14758383035246692TruSeq Adapter, Index 5 (97% over 37bp)
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2808950.1264409437630006No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2740030.1233386066462324No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2239380.10080254922443914No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)