FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141815

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141815
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences158386797
Sequences flagged as poor quality0
Sequence length151
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2260544914.272306422106634No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTCTATCTCGTAT27445301.7328022612894938TruSeq Adapter, Index 25 (97% over 37bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTCTATCGCGTAT10165550.6418180171924306TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3037130.19175398818122447No Hit
GTCAGGACCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGC2709180.17104834817765777No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2496870.15764382178900935No Hit
CCTATACCCAGGTCGGACGACCGATTTGCACGTCAGGACCGCTACGGACC2273700.1435536321881678No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2170620.1370455139641469No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2066510.13047236506714635No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2015800.12727070931297385No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1964050.12400339152006463No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1801030.1137108669480828No Hit
CTCTGGTCCGTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAAT1737050.10967138883425996No Hit
CCGCTACGGACCTCCACCAGAGTTTCCTCTGGCTTCGCCCTGCCCAGGCA1696210.10709289108232929No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)