FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168851491
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA46568282.7579430731825756No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4421450.26185436526586553No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170220.18775196956951953No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3106950.18400488983541163No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATATCCAATCTCGTAT2994740.17735940513548676TruSeq Adapter, Index 9 (97% over 36bp)
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2770290.1640666590264222No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1933170.11448936509539025No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1771980.10494310648402862No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)