FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141883

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141883
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221530679
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA60782122.7437337471438887No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6088250.27482649479894383No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTCGCTGAATCTCGTAT4678010.21116759182596107TruSeq Adapter, Index 3 (97% over 37bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4619390.20852145720187135No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4246450.19168676858522155No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3898820.17599458538200932No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2723530.12294143692847166No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2538570.114592254736871No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)