FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141885

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141885
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences274527729
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66015092.4046783995360994No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6801000.24773453759201133No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGCACATCTCGTAT6286230.2289834262971665TruSeq Adapter, Index 3 (97% over 40bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5414570.19723217103507967No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4863860.17717190236910457No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4000930.14573864777062281No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3264450.11891148525837986No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3111870.11335357675289698No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTACGCACATCGCGTAT2760590.10055778372755927TruSeq Adapter, Index 3 (97% over 40bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)