FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences195837705
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA31765731.6220436202517796No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGACCTAACATCTCGTAT4605540.23517126081517345TruSeq Adapter, Index 9 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730140.19047098208182128No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3023630.15439468104469464No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2722440.13901510947547102No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2052160.10478880969321No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)