FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213119460
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49985542.345423547901257No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6438320.30209911380218396No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCATGCTTAATCTCGTAT5320280.24963839529248055TruSeq Adapter, Index 12 (97% over 37bp)
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4797550.22511083689870462No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4544670.21324519121810837No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4014680.18837697880803564No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2769250.12993886151926248No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2577370.12093546032821217No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)