FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217799958
Sequences flagged as poor quality0
Sequence length151
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA114203085.24348494135155No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCAGCAAATCTCGTAT12073060.554318747848427TruSeq Adapter, Index 6 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7736110.35519336509697585No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5210980.23925532621085263No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5129470.23551290124674862No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4807570.22073328407161583No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCAGCAAATCGCGTAT4115780.18897065168396404TruSeq Adapter, Index 6 (97% over 37bp)
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3036470.1394155457091502No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2896370.13298303758166932No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2697610.12385723233243232No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2683370.12320342137072404No Hit
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2387560.10962169239720422No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)