FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193329196
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA34799841.8000302447851695No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGGTGCGAATCTCGTAT4524940.23405362943732513TruSeq Adapter, Index 14 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4367110.2258898340424485No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3088200.1597379011497053No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3042230.15736009164389222No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2738690.14165941082173641No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1955970.10117302717174699No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)