FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194167455
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28505061.4680657991834936No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTAACTCATCTCGTAT4602040.23701397332524135TruSeq Adapter, Index 22 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4567890.23525518218282257No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3144840.16196535099046336No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3111820.1602647570366517No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2867450.14767922873583525No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGTAACTCATCGCGTAT2150680.11076418548103234TruSeq Adapter, Index 22 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)