FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192968818
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA44339762.297768129563814No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTGGCGATCTCGTAT5916030.3065795842725222TruSeq Adapter, Index 15 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5181690.2685247312858599No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3613630.18726497044719423No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3483830.18053849508473438No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3159670.16373992610557422No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGTGGCGATCGCGTAT2344470.12149475880605747TruSeq Adapter, Index 15 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2272070.11774285729417693No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2021530.10475941247668315No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)