FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196350136
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA41336102.1052239047086783No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATGCGTATCTCGTAT5088630.25916101224396404TruSeq Adapter, Index 2 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4785470.24372124702780953No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3593890.18303475990462262No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3359210.17108264187807845No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3165810.16123289061536478No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTATGCGTATCGCGTAT2136790.10882549121330885TruSeq Adapter, Index 2 (97% over 38bp)
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2007750.1022535578992418No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)