FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences227679056
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA23902661.0498400871795603No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6788550.2981631301212001No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5658420.24852615341131773No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3917690.17207072397559484No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3508760.15410991514300726No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAAGCACAATCTCGTAT3355970.1473991529550263TruSeq Adapter, Index 3 (97% over 37bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2552920.11212801233680449No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)