FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224711016
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA63678162.8337800760065988No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6564130.29211429492179414No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCTGGCGAATCTCGTAT5857770.2606801439587635TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5026540.2236890780646019No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4630170.20604997843096398No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4578430.20374746558931492No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2971460.13223472764681907No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2865750.1275304633930363No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)