FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences198125214
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA55387442.7955775482468375No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAATAGATCTCGTAT9905280.4999505009998374TruSeq Adapter, Index 19 (97% over 38bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5833220.2944208807264682No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4208310.2124065844542129No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020150.20290956001188218No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3622600.1828439665432992No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTTGAATAGATCGCGTAT2599660.13121298130181452TruSeq Adapter, Index 19 (97% over 38bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2356750.11895255290424571No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2306390.11641072599674264No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)