FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184161466
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32956221.7895285433924597No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGCAAGTAATCTCGTAT4351960.23631219356170852TruSeq Adapter, Index 11 (97% over 36bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4295350.2332382606033338No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2955540.160486341914763No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2860160.15530719113628255No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2651760.1439910344762351No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)