FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228119375
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA33956031.488520210087372No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5214360.22858032115860388No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGCGGATATCTCGTAT5182780.22719595825650496TruSeq Adapter, Index 7 (97% over 36bp)
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3727370.16339559057620598No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3585710.15718568403056515No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3418700.14986451720727362No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2289730.10037420100769608No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)