FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236513623
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA35245851.4902249415036868No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4559050.1927605666926002No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCTACCATATCTCGTAT4250210.17970254508341788TruSeq Adapter, Index 22 (97% over 38bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3400850.1437908716150359No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3107350.13138143843832623No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3005820.12708866245729955No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)