FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191494104
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA36833831.9234968195156545No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTACCAGGATCTCGTAT4414640.2305366017953221TruSeq Adapter, Index 23 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3989270.20832338524636768No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3056700.15962371353219312No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2745140.14335376090743765No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2697600.1408711779449878No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)