FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208615193
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA49101062.353666542397993No Hit
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4228570.20269712570742632No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAATGTTCTATCTCGTAT4185330.2006244099393087TruSeq Adapter, Index 25 (97% over 37bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3057150.146544935487992No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2846760.13645985985306447No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2735410.13112228120413072No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)