FastQCFastQC Report
Fri 2 Jun 2023
EGAF00006141985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences157100439
Sequences flagged as poor quality0
Sequence length151
%GC29

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA4140713726.3571109435283No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGGTAATCTCGTAT24347861.5498276233333759TruSeq Adapter, Index 10 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10671660.6792889993133628No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGCGGTAATCGCGTAT9025890.5745299031277691TruSeq Adapter, Index 10 (97% over 37bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8999170.572829080382137No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8043890.5120221210839518No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6057900.3856068155226479No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5016160.31929637064858873No Hit
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4891230.3113441331631161No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3917620.24937040436914373No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3364350.21415280704594342No Hit
CAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2905530.18494728713011427No Hit
CCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2575000.16390788061387912No Hit
AAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA1862070.11852735815715958No Hit
GCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1649700.10500925462086073No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)