FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences200974503
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA66802773.3239425401141554No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACATCCTATCTCGTAT7116770.3541130787122782TruSeq Adapter, Index 7 (97% over 39bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5738870.28555214290043546No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4237670.21085610048753298No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3947450.1964154627117053No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3795890.18887420759040263No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCACATCCTATCGCGTAT2707620.13472455259660476TruSeq Adapter, Index 7 (97% over 39bp)
CTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2424490.12063669588972686No Hit
CCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2390950.11896782747610526No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)