FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences193298076
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA37428651.9363177727645877No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCCTTGATCTCGTAT5985940.30967406007703874TruSeq Adapter, Index 10 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4531420.2344265444214768No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3138210.1623508140867372No Hit
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3002650.15533781101887428No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2794200.14455394786236775No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCGCCTTGATCGCGTAT2527090.1307353933517683TruSeq Adapter, Index 10 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)