FastQCFastQC Report
Fri 16 Dec 2022
EGAF00006141991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00006141991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192943702
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26406581.368615804832023No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACATCATCTCGTAT5035010.26095746830855354TruSeq Adapter, Index 19 (97% over 37bp)
CCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3077310.15949263790947685No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAACATCATCGCGTAT2279260.11813083175941136TruSeq Adapter, Index 19 (97% over 37bp)
CCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2265830.11743477379738468No Hit
CCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2006720.10400546787476898No Hit
CCCCCCCCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1953220.10123263831643492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)